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- W1550671949 abstract "The product of the vaccinia virus I1 gene was characterized biochemically and genetically. This 35-kDa protein is conserved in diverse members of the poxvirus family but shows no homology to nonviral proteins. We show that recombinant I1 binds to both single-stranded and double-stranded DNA in a sequence-nonspecific manner in an electrophoretic mobility shift assay. The protein is expressed at late times during infection, and approximately 700 copies are encapsidated within the virion core. To determine the role of the I1 protein during the viral life cycle, a inducible viral recombinant in which the I1 gene was placed under the regulation of the Escherichia coli lac operator/repressor was constructed. In the absence of isopropyl-beta-D-thiogalactopyranoside, plaque formation was abolished and yields of infectious, intracellular virus were dramatically reduced. Although all phases of gene expression and DNA replication proceeded normally during nonpermissive infections, no mature virions were produced. Electron microscopic analysis confirmed the absence of mature virion assembly but revealed that apparently normal immature virions accumulated. Thus, I1 is an encapsidated DNA-binding protein required for the latest stages of vaccinia virion morphogenesis." @default.
- W1550671949 created "2016-06-24" @default.
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- W1550671949 date "1997-12-01" @default.
- W1550671949 modified "2023-09-26" @default.
- W1550671949 title "The vaccinia virus I1 protein is essential for the assembly of mature virions" @default.
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- W1550671949 doi "https://doi.org/10.1128/jvi.71.12.9285-9294.1997" @default.
- W1550671949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/230231" @default.
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