FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1550872944> ?p ?o ?g. }

• W1550872944 abstract "Abstract Background The early events of the HIV-1 life cycle include entry of the viral core into target cell, assembly of the reverse transcription complex (RTCs) performing reverse transcription, its transformation into integration-competent complexes called pre-integration complexes (PICs), trafficking of complexes into the nucleus, and finally integration of the viral DNA into chromatin. Molecular details and temporal organization of these processes remain among the least investigated and most controversial problems in the biology of HIV. Results To quantitatively evaluate maturation and nuclear translocation of the HIV-1 RTCs, nucleoprotein complexes isolated from the nucleus (nRTC) and cytoplasm (cRTC) of HeLa cells infected with MLV Env-pseudotyped HIV-1 were analyzed by real-time PCR. While most complexes completed reverse transcription in the cytoplasm, some got into the nucleus before completing DNA synthesis. The HIV-specific RNA complexes could get into the nucleus when reverse transcription was blocked by reverse transcriptase inhibitor, although nuclear import of RNA complexes was less efficient than of DNA-containing RTCs. Analysis of the RTC nuclear import in synchronized cells infected in the G2/M phase of the cell cycle showed enrichment in the nuclei of RTCs containing incomplete HIV-1 DNA compared to non-synchronized cells, where RTCs with complete reverse transcripts prevailed. Immunoprecipitation assays identified viral proteins IN, Vpr, MA, and cellular Ini1 and PML associated with both cRTCs and nRTCs, whereas CA was detected only in cRTCs and RT was diminished in nRTCs. Cytoplasmic maturation of the complexes was associated with increased immunoreactivity with anti-Vpr and anti-IN antibodies, and decreased reactivity with antibodies to RT. Both cRTCs and nRTCs carried out endogenous reverse transcription reaction in vitro . In contrast to cRTCs, in vitro completion of reverse transcription in nRTCs did not increase their integration into chromatin. Conclusion These results suggest that RTC maturation occurs predominantly in the cytoplasm. Immature RTCs containing RT and incomplete DNA can translocate into the nucleus during mitosis and complete reverse transcription, but are defective for integration." @default.
• W1550872944 created "2016-06-24" @default.
• W1550872944 creator A5016700147 @default.
• W1550872944 creator A5047632145 @default.
• W1550872944 creator A5059245069 @default.
• W1550872944 creator A5066916338 @default.
• W1550872944 creator A5068425146 @default.
• W1550872944 date "2006-01-12" @default.
• W1550872944 modified "2023-09-30" @default.
• W1550872944 title "Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin" @default.
• W1550872944 cites W1517131856 @default.
• W1550872944 cites W1559159023 @default.
• W1550872944 cites W1584733010 @default.
• W1550872944 cites W1587940136 @default.
• W1550872944 cites W1770932653 @default.
• W1550872944 cites W1972293204 @default.
• W1550872944 cites W1978909186 @default.
• W1550872944 cites W1990957349 @default.
• W1550872944 cites W1994716755 @default.
• W1550872944 cites W1996799677 @default.
• W1550872944 cites W1999686757 @default.
• W1550872944 cites W2000046134 @default.
• W1550872944 cites W2001243226 @default.
• W1550872944 cites W2023247488 @default.
• W1550872944 cites W2047028501 @default.
• W1550872944 cites W2056119728 @default.
• W1550872944 cites W2056884580 @default.
• W1550872944 cites W2058884419 @default.
• W1550872944 cites W2071330468 @default.
• W1550872944 cites W2073273867 @default.
• W1550872944 cites W2076634735 @default.
• W1550872944 cites W2087809591 @default.
• W1550872944 cites W2088907297 @default.
• W1550872944 cites W2099352252 @default.
• W1550872944 cites W2100265029 @default.
• W1550872944 cites W2117098951 @default.
• W1550872944 cites W2118944608 @default.
• W1550872944 cites W2125235624 @default.
• W1550872944 cites W2127741693 @default.
• W1550872944 cites W2128204555 @default.
• W1550872944 cites W2139003099 @default.
• W1550872944 cites W2140613358 @default.
• W1550872944 cites W2141675394 @default.
• W1550872944 cites W2144448640 @default.
• W1550872944 cites W2148690651 @default.
• W1550872944 cites W2149848226 @default.
• W1550872944 cites W2157852305 @default.
• W1550872944 cites W2160935270 @default.
• W1550872944 cites W2169601134 @default.
• W1550872944 cites W2171425914 @default.
• W1550872944 doi "https://doi.org/10.1186/1742-4690-3-4" @default.
• W1550872944 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1360674" @default.
• W1550872944 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16409631" @default.
• W1550872944 hasPublicationYear "2006" @default.
• W1550872944 type Work @default.
• W1550872944 sameAs 1550872944 @default.
• W1550872944 citedByCount "90" @default.
• W1550872944 countsByYear W15508729442012 @default.
• W1550872944 countsByYear W15508729442013 @default.
• W1550872944 countsByYear W15508729442014 @default.
• W1550872944 countsByYear W15508729442015 @default.
• W1550872944 countsByYear W15508729442016 @default.
• W1550872944 countsByYear W15508729442018 @default.
• W1550872944 countsByYear W15508729442020 @default.
• W1550872944 countsByYear W15508729442021 @default.
• W1550872944 countsByYear W15508729442022 @default.
• W1550872944 crossrefType "journal-article" @default.
• W1550872944 hasAuthorship W1550872944A5016700147 @default.
• W1550872944 hasAuthorship W1550872944A5047632145 @default.
• W1550872944 hasAuthorship W1550872944A5059245069 @default.
• W1550872944 hasAuthorship W1550872944A5066916338 @default.
• W1550872944 hasAuthorship W1550872944A5068425146 @default.
• W1550872944 hasBestOaLocation W15508729441 @default.
• W1550872944 hasConcept C104317684 @default.
• W1550872944 hasConcept C126725582 @default.
• W1550872944 hasConcept C138885662 @default.
• W1550872944 hasConcept C1491633281 @default.
• W1550872944 hasConcept C153911025 @default.
• W1550872944 hasConcept C156719811 @default.
• W1550872944 hasConcept C159047783 @default.
• W1550872944 hasConcept C179926584 @default.
• W1550872944 hasConcept C190062978 @default.
• W1550872944 hasConcept C2780114586 @default.
• W1550872944 hasConcept C2780723820 @default.
• W1550872944 hasConcept C29537977 @default.
• W1550872944 hasConcept C41895202 @default.
• W1550872944 hasConcept C54355233 @default.
• W1550872944 hasConcept C552990157 @default.
• W1550872944 hasConcept C60325843 @default.
• W1550872944 hasConcept C67705224 @default.
• W1550872944 hasConcept C83640560 @default.
• W1550872944 hasConcept C86803240 @default.
• W1550872944 hasConcept C95444343 @default.
• W1550872944 hasConceptScore W1550872944C104317684 @default.
• W1550872944 hasConceptScore W1550872944C126725582 @default.
• W1550872944 hasConceptScore W1550872944C138885662 @default.
• W1550872944 hasConceptScore W1550872944C1491633281 @default.
• W1550872944 hasConceptScore W1550872944C153911025 @default.
• W1550872944 hasConceptScore W1550872944C156719811 @default.
• W1550872944 hasConceptScore W1550872944C159047783 @default.

• next