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- W1551114175 abstract "1. DNA polymerase from the mesophile Bacillus licheniformis and the thermophile Bacillus stearothermophilus has been used to study the replication of poly(dA-dT) · poly(dA-dT) and poly(dC) · poly(dG) templates at 37, 45, and 55°C. 2. Incorporation of non-complementary deoxyribonucleoside triphosphates (misincorporation) occurred with both enzymes and both templates. Non-specific incorporation (de novo polynucleotide synthesis, random attachment to existing strands, and tritium exchange of nucleotides) accounted for, at most, a small fraction of the total observed misincorporation. The error rates at 37°C for the complete system were as follows: B. licheniformis: dATP, 161; dCTP, 1830; dGTP, 1360; dTTP, 165; B. stearothermophilus: dATP, 168; dCTP, 11430; dGTP, 1440 dTTP, 167. For both organisms, the error rate for dCTP and dGTP was independent of incubation temperature; the error rate for dATP and dTTP was 5–50-fold greater than that for dCTP or dGTP and increased significantly from 37 to 55°C. 3. The ratio of dATP to dTTP incorporation with the poly(dA-dT) · poly(dA-dT) template was independent of temperature and close to unity. The ratio of dCTP to dGTP incorporation with the poly(dC) · poly(dG) template decreased from approx. 0.2 to 0.05 for the mesophile and from approx. 0.06 to 0.03 for the thermophile as the temperature increased from 37 to 55°C." @default.
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- W1551114175 date "1977-03-01" @default.
- W1551114175 modified "2023-09-30" @default.
- W1551114175 title "DNA polymerase from mesophilic and thermophilic bacteria" @default.
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- W1551114175 doi "https://doi.org/10.1016/0005-2787(77)90336-7" @default.
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