FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1551151508> ?p ?o ?g. }

• W1551151508 endingPage "37" @default.
• W1551151508 startingPage "1228" @default.
• W1551151508 abstract "The biological activity of transforming growth factor-beta 1 (TGF-beta) is governed by dissociation from its latent complex. Immunohistochemical discrimination of active and latent TGF-beta could provide insight into TGF-beta activation in physiological and pathological processes. However, evaluation of immunoreactivity specificity in situ has been hindered by the lack of tissue in which TGF-beta status is known. To provide in situ analysis of antibodies to differentiate between these functional forms, we used xenografts of human tumor cells modified by transfection to overexpress latent TGF-beta or constitutively active TGF-beta. This comparison revealed that, whereas most antibodies did not differentiate between TGF-beta activation status, the immunoreactivity of some antibodies was activation dependent. Two widely used peptide antibodies to the amino-terminus of TGF-beta, LC(1-30) and CC(1-30) showed marked preferential immunoreactivity with active TGF-beta versus latent TGF-beta in cryosections. However, in formalin-fixed, paraffin-embedded tissue, discrimination of active TGF-beta by CC(1-30) was lost and immunoreactivity was distinctly extracellular, as previously reported for this antibody. Similar processing-dependent extracellular localization was found with a neutralizing antibody raised to recombinant TGF-beta. Antigen retrieval recovered cell-associated immunoreactivity of both antibodies. Two antibodies to peptides 78-109 showed mild to moderate preferential immunoreactivity with active TGF-beta only in paraffin sections. LC(1-30) was the only antibody tested that discriminated active from latent TGF-beta in both frozen and paraffin-embedded tissue. Thus, in situ discrimination of active versus latent TGF-beta depends on both the antibody and tissue preparation. We propose that tissues engineered to express a specific form of a given protein provide a physiological setting in which to evaluate antibody reactivity with specific functional forms of a protein." @default.
• W1551151508 created "2016-06-24" @default.
• W1551151508 creator A5000376423 @default.
• W1551151508 creator A5007126253 @default.
• W1551151508 creator A5007563843 @default.
• W1551151508 creator A5030672978 @default.
• W1551151508 creator A5038122136 @default.
• W1551151508 creator A5085308864 @default.
• W1551151508 date "1995-11-01" @default.
• W1551151508 modified "2023-09-23" @default.
• W1551151508 title "Immunohistochemical detection of active transforming growth factor-beta in situ using engineered tissue." @default.
• W1551151508 cites W1479905077 @default.
• W1551151508 cites W1520988136 @default.
• W1551151508 cites W1528159645 @default.
• W1551151508 cites W1581724619 @default.
• W1551151508 cites W1600868878 @default.
• W1551151508 cites W1884021146 @default.
• W1551151508 cites W1889694073 @default.
• W1551151508 cites W1967087287 @default.
• W1551151508 cites W1979877257 @default.
• W1551151508 cites W1982941028 @default.
• W1551151508 cites W1983425803 @default.
• W1551151508 cites W1996719298 @default.
• W1551151508 cites W2000708019 @default.
• W1551151508 cites W2002491528 @default.
• W1551151508 cites W2025691863 @default.
• W1551151508 cites W2027726633 @default.
• W1551151508 cites W2062352917 @default.
• W1551151508 cites W2075263352 @default.
• W1551151508 cites W2097400035 @default.
• W1551151508 cites W2099941826 @default.
• W1551151508 cites W2121126525 @default.
• W1551151508 cites W2122081319 @default.
• W1551151508 cites W2131405836 @default.
• W1551151508 cites W2154636701 @default.
• W1551151508 cites W2161454032 @default.
• W1551151508 cites W2220522056 @default.
• W1551151508 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1869520" @default.
• W1551151508 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7485387" @default.
• W1551151508 hasPublicationYear "1995" @default.
• W1551151508 type Work @default.
• W1551151508 sameAs 1551151508 @default.
• W1551151508 citedByCount "21" @default.
• W1551151508 countsByYear W15511515082012 @default.
• W1551151508 countsByYear W15511515082016 @default.
• W1551151508 crossrefType "journal-article" @default.
• W1551151508 hasAuthorship W1551151508A5000376423 @default.
• W1551151508 hasAuthorship W1551151508A5007126253 @default.
• W1551151508 hasAuthorship W1551151508A5007563843 @default.
• W1551151508 hasAuthorship W1551151508A5030672978 @default.
• W1551151508 hasAuthorship W1551151508A5038122136 @default.
• W1551151508 hasAuthorship W1551151508A5085308864 @default.
• W1551151508 hasConcept C111001777 @default.
• W1551151508 hasConcept C118131993 @default.
• W1551151508 hasConcept C134018914 @default.
• W1551151508 hasConcept C142724271 @default.
• W1551151508 hasConcept C147483822 @default.
• W1551151508 hasConcept C153911025 @default.
• W1551151508 hasConcept C159654299 @default.
• W1551151508 hasConcept C185592680 @default.
• W1551151508 hasConcept C199360897 @default.
• W1551151508 hasConcept C203014093 @default.
• W1551151508 hasConcept C204232928 @default.
• W1551151508 hasConcept C2776174256 @default.
• W1551151508 hasConcept C2778229498 @default.
• W1551151508 hasConcept C28406088 @default.
• W1551151508 hasConcept C41008148 @default.
• W1551151508 hasConcept C55493867 @default.
• W1551151508 hasConcept C71924100 @default.
• W1551151508 hasConcept C86803240 @default.
• W1551151508 hasConceptScore W1551151508C111001777 @default.
• W1551151508 hasConceptScore W1551151508C118131993 @default.
• W1551151508 hasConceptScore W1551151508C134018914 @default.
• W1551151508 hasConceptScore W1551151508C142724271 @default.
• W1551151508 hasConceptScore W1551151508C147483822 @default.
• W1551151508 hasConceptScore W1551151508C153911025 @default.
• W1551151508 hasConceptScore W1551151508C159654299 @default.
• W1551151508 hasConceptScore W1551151508C185592680 @default.
• W1551151508 hasConceptScore W1551151508C199360897 @default.
• W1551151508 hasConceptScore W1551151508C203014093 @default.
• W1551151508 hasConceptScore W1551151508C204232928 @default.
• W1551151508 hasConceptScore W1551151508C2776174256 @default.
• W1551151508 hasConceptScore W1551151508C2778229498 @default.
• W1551151508 hasConceptScore W1551151508C28406088 @default.
• W1551151508 hasConceptScore W1551151508C41008148 @default.
• W1551151508 hasConceptScore W1551151508C55493867 @default.
• W1551151508 hasConceptScore W1551151508C71924100 @default.
• W1551151508 hasConceptScore W1551151508C86803240 @default.
• W1551151508 hasIssue "5" @default.
• W1551151508 hasLocation W15511515081 @default.
• W1551151508 hasOpenAccess W1551151508 @default.
• W1551151508 hasPrimaryLocation W15511515081 @default.
• W1551151508 hasRelatedWork W1551151508 @default.
• W1551151508 hasRelatedWork W1983332906 @default.
• W1551151508 hasRelatedWork W2013366268 @default.
• W1551151508 hasRelatedWork W2074123101 @default.
• W1551151508 hasRelatedWork W2278784660 @default.
• W1551151508 hasRelatedWork W2398608068 @default.

• next