FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1551171898> ?p ?o ?g. }

• W1551171898 endingPage "9352" @default.
• W1551171898 startingPage "9347" @default.
• W1551171898 abstract "The yeast ubiquitin (Ub) conjugating enzyme CDC34 plays a crucial role in the progression of the cell cycle from the G₁to S phase. In an effort to identify proteins that interact with CDC34 we undertook a genetic screen to isolate genes whose increased expression suppressed the cell cycle defect associated with the <i>cdc34-2</i> temperature-sensitive allele. From this screen, the poly-Ub gene <i>UBI4</i> was identified as a moderately strong suppressor. The fact that the overexpression of a gene encoding a single Ub protein could also suppress the <i>cdc34-2</i> allele indicated that suppression was related to the increased abundance of Ub. Ub overexpression was found to suppress two other structurally unrelated <i>cdc34</i> mutations, in addition to the <i>cdc34-2</i> allele. In all three cases, suppression depended on the expression of Ub with an intact carboxyl terminus. Only the <i>cdc34-2</i> allele, however, could be suppressed by Ub with an amino acid substitution at lysine 48 which is known to be involved in multi-Ub chain assembly. Genetic results showing allele specific suppression of <i>cdc34</i> mutations by various Ub derivatives suggested a specific noncovalent interaction between Ub and CDC34. Consistent with this prediction, we have shown by chemical cross-linking the existence of a specific noncovalent Ub binding site on CDC34. Together, these genetic and biochemical experiments indicate that Ub suppression of these <i>cdc34</i> mutations results from the combined contributions of Ub-CDC34 thiol ester formation and a noncovalent interaction between Ub and CDC34 and therefore suggest that the correct positioning of Ub on a surface of the ubiquitin conjugating enzyme is a requirement of enzyme function." @default.
• W1551171898 created "2016-06-24" @default.
• W1551171898 creator A5021908800 @default.
• W1551171898 creator A5054526401 @default.
• W1551171898 creator A5081730087 @default.
• W1551171898 creator A5084678692 @default.
• W1551171898 date "1995-04-01" @default.
• W1551171898 modified "2023-09-29" @default.
• W1551171898 title "Increased Ubiquitin Expression Suppresses the Cell Cycle Defect Associated with the Yeast Ubiquitin Conjugating Enzyme, CDC34 (UBC3)" @default.
• W1551171898 cites W1426117618 @default.
• W1551171898 cites W1481539382 @default.
• W1551171898 cites W1496897161 @default.
• W1551171898 cites W1498526978 @default.
• W1551171898 cites W1502675762 @default.
• W1551171898 cites W1526095797 @default.
• W1551171898 cites W1533591905 @default.
• W1551171898 cites W1542081345 @default.
• W1551171898 cites W1550151833 @default.
• W1551171898 cites W1557920068 @default.
• W1551171898 cites W1558541683 @default.
• W1551171898 cites W1559325314 @default.
• W1551171898 cites W1559808711 @default.
• W1551171898 cites W1566221754 @default.
• W1551171898 cites W1571547690 @default.
• W1551171898 cites W1576912866 @default.
• W1551171898 cites W1590473986 @default.
• W1551171898 cites W1596313018 @default.
• W1551171898 cites W1792328490 @default.
• W1551171898 cites W1838616807 @default.
• W1551171898 cites W1873199501 @default.
• W1551171898 cites W1874958320 @default.
• W1551171898 cites W1910972539 @default.
• W1551171898 cites W1965104714 @default.
• W1551171898 cites W1966263533 @default.
• W1551171898 cites W1978186903 @default.
• W1551171898 cites W1978611748 @default.
• W1551171898 cites W1983155917 @default.
• W1551171898 cites W2013289317 @default.
• W1551171898 cites W2034484654 @default.
• W1551171898 cites W2041770258 @default.
• W1551171898 cites W2049300709 @default.
• W1551171898 cites W206041396 @default.
• W1551171898 cites W2061050157 @default.
• W1551171898 cites W2067397983 @default.
• W1551171898 cites W2068518785 @default.
• W1551171898 cites W2070963888 @default.
• W1551171898 cites W2072280454 @default.
• W1551171898 cites W2115073295 @default.
• W1551171898 cites W2132024812 @default.
• W1551171898 cites W2140014571 @default.
• W1551171898 cites W2175093343 @default.
• W1551171898 cites W2265509655 @default.
• W1551171898 cites W4251551014 @default.
• W1551171898 cites W971227723 @default.
• W1551171898 doi "https://doi.org/10.1074/jbc.270.16.9347" @default.
• W1551171898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7721857" @default.
• W1551171898 hasPublicationYear "1995" @default.
• W1551171898 type Work @default.
• W1551171898 sameAs 1551171898 @default.
• W1551171898 citedByCount "19" @default.
• W1551171898 countsByYear W15511718982020 @default.
• W1551171898 countsByYear W15511718982021 @default.
• W1551171898 crossrefType "journal-article" @default.
• W1551171898 hasAuthorship W1551171898A5021908800 @default.
• W1551171898 hasAuthorship W1551171898A5054526401 @default.
• W1551171898 hasAuthorship W1551171898A5081730087 @default.
• W1551171898 hasAuthorship W1551171898A5084678692 @default.
• W1551171898 hasBestOaLocation W15511718981 @default.
• W1551171898 hasConcept C104317684 @default.
• W1551171898 hasConcept C134459356 @default.
• W1551171898 hasConcept C180754005 @default.
• W1551171898 hasConcept C182561583 @default.
• W1551171898 hasConcept C185592680 @default.
• W1551171898 hasConcept C25602115 @default.
• W1551171898 hasConcept C2776016237 @default.
• W1551171898 hasConcept C2777576037 @default.
• W1551171898 hasConcept C501734568 @default.
• W1551171898 hasConcept C515207424 @default.
• W1551171898 hasConcept C54355233 @default.
• W1551171898 hasConcept C55493867 @default.
• W1551171898 hasConcept C86803240 @default.
• W1551171898 hasConceptScore W1551171898C104317684 @default.
• W1551171898 hasConceptScore W1551171898C134459356 @default.
• W1551171898 hasConceptScore W1551171898C180754005 @default.
• W1551171898 hasConceptScore W1551171898C182561583 @default.
• W1551171898 hasConceptScore W1551171898C185592680 @default.
• W1551171898 hasConceptScore W1551171898C25602115 @default.
• W1551171898 hasConceptScore W1551171898C2776016237 @default.
• W1551171898 hasConceptScore W1551171898C2777576037 @default.
• W1551171898 hasConceptScore W1551171898C501734568 @default.
• W1551171898 hasConceptScore W1551171898C515207424 @default.
• W1551171898 hasConceptScore W1551171898C54355233 @default.
• W1551171898 hasConceptScore W1551171898C55493867 @default.
• W1551171898 hasConceptScore W1551171898C86803240 @default.
• W1551171898 hasIssue "16" @default.
• W1551171898 hasLocation W15511718981 @default.
• W1551171898 hasOpenAccess W1551171898 @default.
• W1551171898 hasPrimaryLocation W15511718981 @default.

• next