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- W1551291503 abstract "Degradation of the labeled glycogen from cells grown overnight in labeled acetate, pyruvate, and lactate confirmed the idea based on enzymatic data that Tetrahymena uses only the glyoxylate cycle in vivo for production of oxaloacetate in the gluconeogenic pathway. Comparison of the incorporation into CO2 and lipid of label from [1-14C] acetate and [2-14C]pyruvate, and [2-14C]acetate and [1-14C]glucose indicated that two pools of acetyl coenzyme A exist in Tetrahymena. One of these, derived most directly from acetate, is associated with lipid synthesis, whereas the other, derived from pyruvate and presumably mitochondrial, is more closely associated with oxidation to CO2. The incorporation of label from [1-14C]glucose and [6-14C]glucose into CO2 and the glycerol and fatty acyl moieties of the lipids was also measured. The ratio of 14C from [1-14C]glucose to that from [6-14C]glucose was about 0.5 for each of these products. The enrichment of label from [6-14C]glucose relative to that from [1-14C]glucose in these products indicates that transaldolase plays a role in carbon flow in the glycolytic and glyconeogenic pathways of Tetrahymena." @default.
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- W1551291503 date "1972-05-01" @default.
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- W1551291503 title "Metabolic Pathways in Tetrahymena" @default.
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- W1551291503 doi "https://doi.org/10.1016/s0021-9258(19)45261-7" @default.
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