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• W155180017 abstract "Ankaferd blood stopper (ABS) is comprised of a mixture of the plants Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. ABS is used as a topical hemostatic agent due to its antihemorrhagic effect, yet its hemostatic mechanism of action remains to be investigated. ABS does not affect the levels of coagulation factors II, V, VII, VIII, IX, X, XI and XII. The aim of this study was to investigate the effects of ABS on endothelium and immune response. As such, we evaluated changes in endothelial cell protein C receptor (EPCR) and plasminogen activator inhibitor type-1 (PAI-1) expression inside human umbilical vein endothelial cells (HUVECs) in the presence and absence of lipopolysaccharides (LPSs).We exposed HUVECs to 10 μL and 100 μL of ABS for 5 min, 25 min, 50 min, 6 h, and 24 h. Additionally, 10 μg mL-1 of LPS was administered for 1 h to observe the effects of LPS challenge on HUVECs, and then the cells were treated with ABS for 5 min, 25 min, 50 min, and 6 h to observe the effects of ABS on HUVECs. Total RNA was isolated from HUVECs and then the level of expression of EPCR and PAI-1 mRNA was measured.Cells were microscopically observed to arise from the surface and adhere to each other following the administration of ABS to HUVECs. Additionally, after 24 h the cells had normal growth and physiology, which suggests that the adhesive cellular effects of ABS might be reversible. ABS had a negative effect on EPCR and PAI-1 expression; the effect in response to 100 µL was greater than that to 10 µL. EPCR and PAI-1 expression increased over time in response to LPS and 10 µL of ABS. EPCR and PAI-1 expression was very low during the first hour of exposure to LPS and 100 µL of ABS, but after 6 h increased to levels similar to those observed in response to LPS and 10 µL of ABS.It was observed that ABS had dual diverse dynamic reversible effects on EPCR and PAI-1 expression in HUVECs, which were dependent on dose and concentration. ABS might play a role in numerous cellular mechanisms, in addition to having hemostatic effects.None declared.Amaç: Ankaferd, Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum ve Urtica dioica bitkilerindenoluşan bir karışımdır. Kanamayı durdurucu etkisinden dolayı topikal hemostatik ajan olarak kullanılmaktadır.Hemostatik çalışma mekanizması araştırılmaktadır. Koagülasyon faktörleri II, V, VII, VIII, IX, X, XI ve XII seviyeleriAnkaferd’den etkilenmemektedir. Çalışmamızda Ankaferd’in endotel üzerindeki ve immün yanıttaki etkisini araştırmakamacıyla, İnsan Umbilical Ven Endotel Hücreleri (HUVEC) kullanılarak Endotelyal Protein C Reseptörü (EPCR) vePlazminojen Aktivatör İnhibitör (PAI-1) gen ekspresyonları üzerindeki etkisi araştırılmış ve farklı doz ve zamanlardaoluşturduğu değişikliklerin incelenmesi, lipopolisakkarit (LPS) muamelesinden sonra EPCR ve PAI-1 ekspresyonlarıüzerindeki olası etkisinin gösterilmesi amaçlanmıştır.Gereç ve Yöntemler: HUVEC’lere 10 µl ve 100 µl konsantrasyonlarda Ankaferd 5 dk, 25 dk, 50 dk, 6 saat ve 24saat süreyle uygulanmıştır. Ayrıca immün yanıt üzerindeki etkisinin incelenebilmesi için 10 µg/ml LPS ile 1 saatlikmuamelenin ardından hücreler 10 µl ve 100 µl Ankaferd’le birlikte 5 dk, 25 dk, 50 dk ve 6 saatlik sürelerde maruzbırakılmıştır. HUVEC hücrelerinden RNA izolasyonu yapılmış; EPCR ve PAI-1 gen ekspresyonları incelenmiştir.Bulgular: Yapılan mikroskobik incelemede Ankaferd uygulamasıyla hücrelerin yüzeyden kalkıp toplanarak birbirlerineyapıştıkları ve 24 saatin içerisinde büyüme ve gelişmelerinin normale döndüğü gözlenmiştir. Bu, Ankaferd’in etkisininzamanla azaldığı anlamına gelebilir. Ankaferd muamelesiyle başlangıçta gözlenen EPCR ve PAI-1 ekspresyonundakideğişim zamanla azalmıştır. Ayrıca ekspresyonlardaki hızlı değişim göz önüne alındığında Ankaferd’in artankonsantrasyonlarda etkisini daha da fazla gösterdiği söylenebilir. Böylece Ankaferd’in EPCR ve PAI-1 üzerinde hemdoz, hem de zamana bağlı etkisi belirlenmiştir. LPS muamelesi sonrasında da hücreler üzerinde Ankaferd benzer bir etkigöstermekte, doz arttıkça daha da etkili hale gelmekte ve zamanla etkisini yitirmeye başlamaktadır.Sonuç: Ankaferd’in HUVEC’lerde EPCR ve PAI-1 ekspresyonu üzerinde doz ve konsantrasyona bağımlı etkisinin varlığısaptanmış olup, hemostatik etkileri yanısıra hücresel birçok mekanizmayı da etkileyebileceği düşünülmektedir." @default.
• W155180017 created "2016-06-24" @default.
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• W155180017 date "2011-08-01" @default.
• W155180017 modified "2023-10-17" @default.
• W155180017 title "Dual diverse dynamic reversible actions of Ankaferd on EPCR and PAI-1 inside vascular endothelial cells with and without LPS" @default.
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• W155180017 doi "https://doi.org/10.5152/tjh.2011.41" @default.
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