FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1552201170> ?p ?o ?g. }

• W1552201170 endingPage "64" @default.
• W1552201170 startingPage "59" @default.
• W1552201170 abstract "ABSTRACT Abnormal fibrillization of amyloidogenic peptides/proteins has been linked to various neurodegenerative diseases such as Alzheimer's and Parkinson's disease as well as with type‐II diabetes mellitus. The kinetics of protein fibrillization is commonly studied by using a fluorescent dye Thioflavin T (ThT) that binds to protein fibrils and exerts increased fluorescence intensity in bound state. Recently, it has been demonstrated that several low‐molecular weight compounds like Basic Blue 41, Basic Blue 12, Azure C, and Tannic acid interfere with the fluorescence of ThT bound to Alzheimers' amyloid‐ β fibrils and cause false positive results during the screening of fibrillization inhibitors. In the current study, we demonstrated that the same selected substances also decrease the fluorescence signal of ThT bound to insulin fibrils already at submicromolar or micromolar concentrations. Kinetic experiments show that unlike to true inhibitors, these compounds did neither decrease the fibrillization rate nor increase the lag‐period. Absence of soluble insulin in the end of the experiment confirmed that these compounds do not disaggregate the insulin fibrils and, thus, are not fibrillization inhibitors at concentrations studied. Our results show that interference with ThT test is a general phenomenon and more attention has to be paid to interpretation of kinetic results of protein fibrillization obtained by using fluorescent dyes. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd." @default.
• W1552201170 created "2016-06-24" @default.
• W1552201170 creator A5014821902 @default.
• W1552201170 creator A5065013688 @default.
• W1552201170 creator A5080223742 @default.
• W1552201170 creator A5009376732 @default.
• W1552201170 date "2011-11-14" @default.
• W1552201170 modified "2023-09-30" @default.
• W1552201170 title "Interference of low-molecular substances with the thioflavin-T fluorescence assay of amyloid fibrils" @default.
• W1552201170 cites W1973897761 @default.
• W1552201170 cites W2005279391 @default.
• W1552201170 cites W2015389516 @default.
• W1552201170 cites W2018880174 @default.
• W1552201170 cites W2048031945 @default.
• W1552201170 cites W2057436204 @default.
• W1552201170 cites W2059988328 @default.
• W1552201170 cites W2062609017 @default.
• W1552201170 cites W2068693169 @default.
• W1552201170 cites W2076713412 @default.
• W1552201170 cites W2082501707 @default.
• W1552201170 cites W2084527204 @default.
• W1552201170 cites W2108305553 @default.
• W1552201170 cites W2115537581 @default.
• W1552201170 cites W2143451161 @default.
• W1552201170 cites W2144535585 @default.
• W1552201170 cites W2154255598 @default.
• W1552201170 cites W2314611309 @default.
• W1552201170 doi "https://doi.org/10.1002/psc.1416" @default.
• W1552201170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22083646" @default.
• W1552201170 hasPublicationYear "2011" @default.
• W1552201170 type Work @default.
• W1552201170 sameAs 1552201170 @default.
• W1552201170 citedByCount "32" @default.
• W1552201170 countsByYear W15522011702012 @default.
• W1552201170 countsByYear W15522011702013 @default.
• W1552201170 countsByYear W15522011702014 @default.
• W1552201170 countsByYear W15522011702015 @default.
• W1552201170 countsByYear W15522011702016 @default.
• W1552201170 countsByYear W15522011702017 @default.
• W1552201170 countsByYear W15522011702018 @default.
• W1552201170 countsByYear W15522011702019 @default.
• W1552201170 countsByYear W15522011702020 @default.
• W1552201170 countsByYear W15522011702021 @default.
• W1552201170 countsByYear W15522011702022 @default.
• W1552201170 countsByYear W15522011702023 @default.
• W1552201170 crossrefType "journal-article" @default.
• W1552201170 hasAuthorship W1552201170A5009376732 @default.
• W1552201170 hasAuthorship W1552201170A5014821902 @default.
• W1552201170 hasAuthorship W1552201170A5065013688 @default.
• W1552201170 hasAuthorship W1552201170A5080223742 @default.
• W1552201170 hasConcept C121332964 @default.
• W1552201170 hasConcept C12554922 @default.
• W1552201170 hasConcept C126322002 @default.
• W1552201170 hasConcept C136238340 @default.
• W1552201170 hasConcept C150394285 @default.
• W1552201170 hasConcept C178790620 @default.
• W1552201170 hasConcept C179104552 @default.
• W1552201170 hasConcept C185592680 @default.
• W1552201170 hasConcept C27523624 @default.
• W1552201170 hasConcept C2777633098 @default.
• W1552201170 hasConcept C2778896982 @default.
• W1552201170 hasConcept C2779134260 @default.
• W1552201170 hasConcept C2779155187 @default.
• W1552201170 hasConcept C2779281246 @default.
• W1552201170 hasConcept C502032728 @default.
• W1552201170 hasConcept C55493867 @default.
• W1552201170 hasConcept C62520636 @default.
• W1552201170 hasConcept C71924100 @default.
• W1552201170 hasConcept C86803240 @default.
• W1552201170 hasConcept C91881484 @default.
• W1552201170 hasConceptScore W1552201170C121332964 @default.
• W1552201170 hasConceptScore W1552201170C12554922 @default.
• W1552201170 hasConceptScore W1552201170C126322002 @default.
• W1552201170 hasConceptScore W1552201170C136238340 @default.
• W1552201170 hasConceptScore W1552201170C150394285 @default.
• W1552201170 hasConceptScore W1552201170C178790620 @default.
• W1552201170 hasConceptScore W1552201170C179104552 @default.
• W1552201170 hasConceptScore W1552201170C185592680 @default.
• W1552201170 hasConceptScore W1552201170C27523624 @default.
• W1552201170 hasConceptScore W1552201170C2777633098 @default.
• W1552201170 hasConceptScore W1552201170C2778896982 @default.
• W1552201170 hasConceptScore W1552201170C2779134260 @default.
• W1552201170 hasConceptScore W1552201170C2779155187 @default.
• W1552201170 hasConceptScore W1552201170C2779281246 @default.
• W1552201170 hasConceptScore W1552201170C502032728 @default.
• W1552201170 hasConceptScore W1552201170C55493867 @default.
• W1552201170 hasConceptScore W1552201170C62520636 @default.
• W1552201170 hasConceptScore W1552201170C71924100 @default.
• W1552201170 hasConceptScore W1552201170C86803240 @default.
• W1552201170 hasConceptScore W1552201170C91881484 @default.
• W1552201170 hasIssue "1" @default.
• W1552201170 hasLocation W15522011701 @default.
• W1552201170 hasLocation W15522011702 @default.
• W1552201170 hasOpenAccess W1552201170 @default.
• W1552201170 hasPrimaryLocation W15522011701 @default.
• W1552201170 hasRelatedWork W1980082404 @default.
• W1552201170 hasRelatedWork W2028194071 @default.
• W1552201170 hasRelatedWork W2028857921 @default.

• next