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- W1552235071 abstract "The rhizosphere of cereals grown in different regions of Greece is colonized by nitrogen-fixing bacteria belonging to the genus of Azospirillum and Pseudomonas (Venieraki, A, Dimou M, Pergalis P, Kefalogianni I, Chatzipavlidis I, Katinakis P. The genetic diversity of culturable nitrogen-fixing bacteria in the rhizosphere of wheat. Microb Ecol 2011a;61:277–285; Venieraki, A, Dimou M, Vezyri E, Kefalogianni I, Argyris N, Liara G, et al. Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat and wheat. J Microbiol 2011b;49:525–544). Pairwise comparisons of the 16S rRNA gene sequence revealed that several isolates previously described as Azospirillum zeae differed in their 16S rRNA gene nucleotide sequences more than the recently set upper limit of 1.3% for species discrimination. In the present study, the genetic diversity of these isolates is compared to type strain A. zeae N7 by applying phylogenetic reconstruction of 16S rRNA gene, sequencing internal transcribed spacer 1 (ITS1) region and repetitive element palindromic (Rep)-PCR analysis. The phylogenetic trees inferred from the 16S rRNA gene and ITS1 sequences demonstrate that most of the isolates represent novel sublines within the genus Azospirillum. Rep-PCR fingerprinting revealed that the banding pattern of all isolates was different from that observed for A. zeae N7. Furthermore, the swarming migration phenotype varied significantly among the six Azospirillum strains and was dependant on environmental conditions. Interestingly, in some cases, the inhibitory effect of increasing NaCl concentration on swarming migration was partially alleviated by elevated temperature." @default.
- W1552235071 created "2016-06-24" @default.
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- W1552235071 date "2013-03-18" @default.
- W1552235071 modified "2023-10-13" @default.
- W1552235071 title "Phylogenetic Analysis of <i>Azospirillum</i> Species Isolated from the Rhizosphere of Field‐Grown Wheat Based on Genetic and Phenotypic Features" @default.
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- W1552235071 doi "https://doi.org/10.1002/9781118297674.ch19" @default.
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