FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1553415829> ?p ?o ?g. }

• W1553415829 endingPage "13275" @default.
• W1553415829 startingPage "13268" @default.
• W1553415829 abstract "The covalent ligation of the 8.6-kDa protein ubiquitin to histones within transcriptionally poised regions is believed to participate in the localized regulation of chromatin structure. This unique post-translational modification is thought to be distinct from similar cytosolic reactions in requiring ubiquitin-activating enzyme (E1) and one or more putative ubiquitin carrier proteins (E2) but not isopeptide ligase (E3). Apparently homogeneous preparations of the E2 isozymes were tested for their ability to catalyze the E3-independent conjugation of ubiquitin to linker histone H1 and core histones H2A, H2B, H3, and H4 in the presence of catalytic amounts of E1. Significant rates of nonprocessive core histone monoubiquitination were catalyzed by the E2(14kDa), E2(20kDa), and E2(32kDa) isozymes but not by either E2(17kDa) or E2(24kDa). The former three E2 isozymes also supported slow rates of direct multiple ubiquitination to secondary ligation sites on the histones. Rate studies for the monoubiquitination of H2A and H2B revealed that: 1) E2(14kDa) catalyzed a second order reaction with respect to histone concentration; 2) E2(32kDa)-mediated ligation proceeded by hyperbolic kinetics, yielding Km values of 2.8 and 12 microM for H2A and H2B, respectively; and 3) E2(20kDa) exhibited complex kinetics composed of both second order and hyperbolic pathways, the latter having Km values of 0.83 and 1.5 microM for H2A and H2B, respectively. Pulse-chase kinetics suggested that both ubiquitin thiol esters formed to E2(20kDa) were catalytically competent in H2A ligation. The active E2 isozymes also catalyzed the processive multiple ubiquitination of calf thymus H1. Other rate studies determined that Kd values for binding of the active E2 species to E1 ternary complex were 0.1 nM for E2(14kDa), 0.4 nM for E2(32kDa), and 3.6 nM for E2(20kDa). The data indicate that E2(20kDa) and E2(32kDa) are specific but mechanistically distinct ligation enzymes responsible for the conjugation of ubiquitin to nucleosomal proteins." @default.
• W1553415829 created "2016-06-24" @default.
• W1553415829 creator A5012442571 @default.
• W1553415829 creator A5034615748 @default.
• W1553415829 creator A5081365529 @default.
• W1553415829 date "1988-09-01" @default.
• W1553415829 modified "2023-09-27" @default.
• W1553415829 title "Functional diversity among putative E2 isozymes in the mechanism of ubiquitin-histone ligation." @default.
• W1553415829 cites W1479851678 @default.
• W1553415829 cites W1495218540 @default.
• W1553415829 cites W1498526978 @default.
• W1553415829 cites W1513849811 @default.
• W1553415829 cites W1526095797 @default.
• W1553415829 cites W1549578642 @default.
• W1553415829 cites W1561767449 @default.
• W1553415829 cites W1564518002 @default.
• W1553415829 cites W1566443303 @default.
• W1553415829 cites W1585805915 @default.
• W1553415829 cites W1628512331 @default.
• W1553415829 cites W1775749144 @default.
• W1553415829 cites W1968035033 @default.
• W1553415829 cites W1977093640 @default.
• W1553415829 cites W1983970621 @default.
• W1553415829 cites W1992356183 @default.
• W1553415829 cites W1998475203 @default.
• W1553415829 cites W1999406663 @default.
• W1553415829 cites W2001274114 @default.
• W1553415829 cites W2001714494 @default.
• W1553415829 cites W2004215286 @default.
• W1553415829 cites W2009159113 @default.
• W1553415829 cites W2013289317 @default.
• W1553415829 cites W2017048095 @default.
• W1553415829 cites W2018474558 @default.
• W1553415829 cites W2035340043 @default.
• W1553415829 cites W2042466358 @default.
• W1553415829 cites W2056793770 @default.
• W1553415829 cites W2065362756 @default.
• W1553415829 cites W2069385831 @default.
• W1553415829 cites W2075610152 @default.
• W1553415829 cites W2081864633 @default.
• W1553415829 cites W2083370021 @default.
• W1553415829 cites W2089840805 @default.
• W1553415829 cites W2094887095 @default.
• W1553415829 cites W2100837269 @default.
• W1553415829 cites W2101917549 @default.
• W1553415829 cites W2131097094 @default.
• W1553415829 cites W2166174738 @default.
• W1553415829 cites W22154000 @default.
• W1553415829 cites W2417705698 @default.
• W1553415829 cites W303857720 @default.
• W1553415829 cites W4242845182 @default.
• W1553415829 cites W4254147892 @default.
• W1553415829 cites W81961228 @default.
• W1553415829 doi "https://doi.org/10.1016/s0021-9258(18)37700-7" @default.
• W1553415829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2843516" @default.
• W1553415829 hasPublicationYear "1988" @default.
• W1553415829 type Work @default.
• W1553415829 sameAs 1553415829 @default.
• W1553415829 citedByCount "96" @default.
• W1553415829 countsByYear W15534158292012 @default.
• W1553415829 countsByYear W15534158292013 @default.
• W1553415829 countsByYear W15534158292015 @default.
• W1553415829 countsByYear W15534158292016 @default.
• W1553415829 countsByYear W15534158292017 @default.
• W1553415829 countsByYear W15534158292018 @default.
• W1553415829 countsByYear W15534158292020 @default.
• W1553415829 countsByYear W15534158292021 @default.
• W1553415829 countsByYear W15534158292022 @default.
• W1553415829 countsByYear W15534158292023 @default.
• W1553415829 crossrefType "journal-article" @default.
• W1553415829 hasAuthorship W1553415829A5012442571 @default.
• W1553415829 hasAuthorship W1553415829A5034615748 @default.
• W1553415829 hasAuthorship W1553415829A5081365529 @default.
• W1553415829 hasBestOaLocation W15534158291 @default.
• W1553415829 hasConcept C104317684 @default.
• W1553415829 hasConcept C111472728 @default.
• W1553415829 hasConcept C119795356 @default.
• W1553415829 hasConcept C138885662 @default.
• W1553415829 hasConcept C153911025 @default.
• W1553415829 hasConcept C179437574 @default.
• W1553415829 hasConcept C181199279 @default.
• W1553415829 hasConcept C185592680 @default.
• W1553415829 hasConcept C25602115 @default.
• W1553415829 hasConcept C552990157 @default.
• W1553415829 hasConcept C55493867 @default.
• W1553415829 hasConcept C64927066 @default.
• W1553415829 hasConcept C70721500 @default.
• W1553415829 hasConcept C86803240 @default.
• W1553415829 hasConcept C89611455 @default.
• W1553415829 hasConcept C95444343 @default.
• W1553415829 hasConceptScore W1553415829C104317684 @default.
• W1553415829 hasConceptScore W1553415829C111472728 @default.
• W1553415829 hasConceptScore W1553415829C119795356 @default.
• W1553415829 hasConceptScore W1553415829C138885662 @default.
• W1553415829 hasConceptScore W1553415829C153911025 @default.
• W1553415829 hasConceptScore W1553415829C179437574 @default.
• W1553415829 hasConceptScore W1553415829C181199279 @default.
• W1553415829 hasConceptScore W1553415829C185592680 @default.

• next