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- W1553602118 abstract "Publisher Summary The anthranilate synthetase complex is obtained from crude extracts of S. typhimurium strain trp E171 and free anthranilate synthetase and anthranilate-PRtransferase from crude extracts of strains trp BEDC43 and trp A703, respectively. The cells are harvested by centrifugation, washed twice, and then suspended in volumes of 0.1 M potassium phosphate buffer, pH 7.8, containing 1 mM 2-mercaptoethanol. Crude extracts are immediately prepared by ultrasonic disintegration all procedures being carried out at 4°C. Complexed anthranilate synthetase activity is assayed using the spectrophotofluorometric method of Henderson as is anthranilate-PRtransferase activity except that in both cases a 3-ml volume is used and 2-mercaptoethanol is substituted for dithiothreitol. Free anthranilate synthetase activity is assayed by the method of Zalkin and Kling. Cborismic acid is prepared by the method of Gibson. Protein concentrations are determined using the method of Lowry. One unit of enzyme activity is defined as the production or disappearance of 1 nmole of anthranilic acid per minute at 24–25 °C." @default.
- W1553602118 created "2016-06-24" @default.
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- W1553602118 date "1974-01-01" @default.
- W1553602118 modified "2023-09-27" @default.
- W1553602118 title "[40]l-Tryptophan-agarose in the purification of an anthranilate synthetase complex" @default.
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- W1553602118 doi "https://doi.org/10.1016/s0076-6879(74)34043-8" @default.
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