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- W1553662387 abstract "Aim In rat middle cerebral arteries myogenic tone is normally suppressed by a basal release of NO as inhibition of nitric oxide synthase (NOS) causes both vasoconstriction and smooth muscle depolarization associated with oscillations in tension and membrane potential (vasomotion). We wished to characterise the cellular mechanisms which underpin the vasoconstrictor effect of inhibitors of NOS by simultaneously measuring smooth muscle cell (SMC) membrane potential and tension or SMC [Ca2+] and tension Methods Segments of the middle cerebral artery from male Wistar rats were mounted in a wire myograph. SMC membrane potential (Em) was recorded with sharp glass microelectrodes. SMC [Ca2+] was recorded on a confocal microscope with vessels loaded with fluo-4. Results Inhibition of NOS with L-NAME caused SMC depolarization associated with constriction and the development of vasomotion temporally linked to SMC [Ca2+]. This effect was mimicked by the blocker of guanylyl cyclase, ODQ and the BKCa blocker, iberiotoxin. Relaxation and hyperpolarization produced by the NO donor DEA-NONOate was inhibited by iberiotoxin. L-NAME-induced constriction and oscillations in SMC [Ca2+] were abolished by the L-type Ca channel inhibitor, nifedipine. Discussion In rat middle cerebral arteries inhibition of NOS with L-NAME induces vasoconstriction and depolarization involving the entry of Ca2+. L-NAME also induces vasomotion which is underpinned by oscillations in Em and SMC [Ca2+]. Blockade of BKCa mimics the effects of L-NAME suggesting that NO normally suppresses myogenic tone by activation of SMC BKCa channels. Supported by the British Heart Foundation" @default.
- W1553662387 created "2016-06-24" @default.
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- W1553662387 date "2006-03-01" @default.
- W1553662387 modified "2023-09-23" @default.
- W1553662387 title "NO can suppress myogenic tone in rat middle cerebral arteries by activating BK Ca" @default.
- W1553662387 doi "https://doi.org/10.1096/fasebj.20.5.a1113-b" @default.
- W1553662387 hasPublicationYear "2006" @default.
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