Matches in SemOpenAlex for { <https://semopenalex.org/work/W1553824648> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W1553824648 endingPage "6" @default.
- W1553824648 startingPage "601" @default.
- W1553824648 abstract "The effectiveness of separation of murine X- and Y-bearing sperm by free-flow electrophoresis was evaluated by the polymerase chain reaction (PCR). The ratio of X- and Y-bearing sperm from cauda epididymis was analyzed before and after free-flow electrophoresis. A Y-chromosome-specific sequence (pY353/B) and an autosomal sequence (myogenin) were used to estimate the ratio between X- and Y-sperm in the separated fractions. Cauda epididymal mice sperm were separated into two peak fractions under the electric field. Each peak fraction contained sperm of normal shape, however, the motility of the sperm was extremely diminished after separation by electrophoresis. DNA was extracted from 10(4) sperm from each fraction and from the unseparated sperm, and Y-chromosome specific PCR was performed. The PCR experiment revealed that fraction No. 16 (the peak near the cathode) was a Y-sperm rich fraction, whereas fraction No. 22 (the peak near the anode) was a Y-sperm poor one. These results suggested that murine X- and Y-sperm could be successfully separated by free-flow electrophoresis. Analysis of the chromosome-specific sequence by PCR was demonstrated to be a direct and adequate method to evaluate the separation of X- and Y-sperm." @default.
- W1553824648 created "2016-06-24" @default.
- W1553824648 creator A5019425516 @default.
- W1553824648 creator A5022924573 @default.
- W1553824648 creator A5045634430 @default.
- W1553824648 creator A5051437451 @default.
- W1553824648 creator A5081465675 @default.
- W1553824648 date "1992-06-01" @default.
- W1553824648 modified "2023-10-18" @default.
- W1553824648 title "Separation of X- and Y-chromosome-bearing murine sperm by free-flow electrophoresis: evaluation of separation using PCR." @default.
- W1553824648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1369423" @default.
- W1553824648 hasPublicationYear "1992" @default.
- W1553824648 type Work @default.
- W1553824648 sameAs 1553824648 @default.
- W1553824648 citedByCount "3" @default.
- W1553824648 countsByYear W15538246482021 @default.
- W1553824648 crossrefType "journal-article" @default.
- W1553824648 hasAuthorship W1553824648A5019425516 @default.
- W1553824648 hasAuthorship W1553824648A5022924573 @default.
- W1553824648 hasAuthorship W1553824648A5045634430 @default.
- W1553824648 hasAuthorship W1553824648A5051437451 @default.
- W1553824648 hasAuthorship W1553824648A5081465675 @default.
- W1553824648 hasConcept C101660505 @default.
- W1553824648 hasConcept C104317684 @default.
- W1553824648 hasConcept C116084860 @default.
- W1553824648 hasConcept C153911025 @default.
- W1553824648 hasConcept C181199279 @default.
- W1553824648 hasConcept C185592680 @default.
- W1553824648 hasConcept C23347341 @default.
- W1553824648 hasConcept C2778093475 @default.
- W1553824648 hasConcept C2780246931 @default.
- W1553824648 hasConcept C2781087480 @default.
- W1553824648 hasConcept C2781254305 @default.
- W1553824648 hasConcept C35859425 @default.
- W1553824648 hasConcept C40250595 @default.
- W1553824648 hasConcept C43617362 @default.
- W1553824648 hasConcept C49105822 @default.
- W1553824648 hasConcept C54355233 @default.
- W1553824648 hasConcept C55493867 @default.
- W1553824648 hasConcept C86803240 @default.
- W1553824648 hasConceptScore W1553824648C101660505 @default.
- W1553824648 hasConceptScore W1553824648C104317684 @default.
- W1553824648 hasConceptScore W1553824648C116084860 @default.
- W1553824648 hasConceptScore W1553824648C153911025 @default.
- W1553824648 hasConceptScore W1553824648C181199279 @default.
- W1553824648 hasConceptScore W1553824648C185592680 @default.
- W1553824648 hasConceptScore W1553824648C23347341 @default.
- W1553824648 hasConceptScore W1553824648C2778093475 @default.
- W1553824648 hasConceptScore W1553824648C2780246931 @default.
- W1553824648 hasConceptScore W1553824648C2781087480 @default.
- W1553824648 hasConceptScore W1553824648C2781254305 @default.
- W1553824648 hasConceptScore W1553824648C35859425 @default.
- W1553824648 hasConceptScore W1553824648C40250595 @default.
- W1553824648 hasConceptScore W1553824648C43617362 @default.
- W1553824648 hasConceptScore W1553824648C49105822 @default.
- W1553824648 hasConceptScore W1553824648C54355233 @default.
- W1553824648 hasConceptScore W1553824648C55493867 @default.
- W1553824648 hasConceptScore W1553824648C86803240 @default.
- W1553824648 hasIssue "3" @default.
- W1553824648 hasLocation W15538246481 @default.
- W1553824648 hasOpenAccess W1553824648 @default.
- W1553824648 hasPrimaryLocation W15538246481 @default.
- W1553824648 hasRelatedWork W1553824648 @default.
- W1553824648 hasRelatedWork W1967747576 @default.
- W1553824648 hasRelatedWork W1969242820 @default.
- W1553824648 hasRelatedWork W2010754152 @default.
- W1553824648 hasRelatedWork W2011522896 @default.
- W1553824648 hasRelatedWork W2080671555 @default.
- W1553824648 hasRelatedWork W2102034346 @default.
- W1553824648 hasRelatedWork W2110267269 @default.
- W1553824648 hasRelatedWork W4200516424 @default.
- W1553824648 hasRelatedWork W4214925635 @default.
- W1553824648 hasVolume "9" @default.
- W1553824648 isParatext "false" @default.
- W1553824648 isRetracted "false" @default.
- W1553824648 magId "1553824648" @default.
- W1553824648 workType "article" @default.