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- W1553969894 abstract "Drosophila melanogaster is often used to understand genetic mechanisms because of the wealth of know ledge obtained concerning the organism's molecular functioning. One of the genes contained in the Drosophila's repertoire is the Cyp6a8 gene which is the subject of the present investigation. This gene codes for an enzyme that is a member of the cytochrome P450 (CYP) family. CYPs are found in all organisms, and they are in the metabolism of various xenobiotic (foreign) and endogenous compounds. In insects, CYPs playa major role in conferring insecticide resistance. In humans and other mammals, xenobiotic compounds often induce a variety of Cyp genes to produce enzymes that in some case metabolize the inducing compounds. The objective of this investigation has been to examine whether DDT, one of the most potent insecticides, is capable of inducing an insect Cyp gene. For this purpose, two transgenic lines of Drosophila melanogaster carrying the fIrefly lueiferase (lue) reporter gene were used. These two lines, 0.2 lue and 0.8lue, have the lue reporter gene under the control of 0.2or 0.8kb upstream DNA of Cyp6a8, respectively. In these transgenic lines, the level of lue activity is generally proportional to the promoter activity of the Cyp6a8 upstream DNA. Three-day old adult female transgenic flies were allowed to feed on normal (control) and DDT (10 Ilg/mL) containing medium for 24 hours. Results showed that DDT induced lue activity in both transgenic lines. The average level of induction in 0.2 lue and 0.8 lue lines was about 4.5and 2.5fold, respectively. These results suggest that the 0.2kb upstream DNA of Cyp6a8 has cisacting elements for DDT induction. The 0.8kb DNA also has these cis elements, but the extra 0.6kb DNA somehow intereferes with maximum level of induction. However, the 0.8 lue line showed greater basal induction indicating that the extra DNA also was able to produce more enzymes." @default.
- W1553969894 created "2016-06-24" @default.
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- W1553969894 date "2003-01-01" @default.
- W1553969894 modified "2023-09-24" @default.
- W1553969894 title "The Effect of DDT on the Cytochrome P450 gene, Cyp6a8, of Drosophila Melanogaster" @default.
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