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• W155404871 abstract "Spinning-disk confocal microscopy is an imaging technique that combines the out-of-focus light rejection of confocal microscopy with the high sensitivity of wide-field microscopy. Because of its unique features, it is well suited to high-resolution imaging of yeast and other small cells. Elimination of out-of-focus light significantly improves the image contrast and signal-to-noise ratio, making it easier to resolve and quantitate small, dim structures in the cell. These features make spinning-disk confocal microscopy an excellent technique for studying protein localization and dynamics in yeast. In this review, I describe the rationale behind using spinning-disk confocal imaging for yeast, hardware considerations when assembling a spinning-disk confocal scope, and methods for strain preparation and imaging. In particular, I discuss choices of objective lens and camera, choice of fluorescent proteins for tagging yeast genes, and methods for sample preparation." @default.
• W155404871 created "2016-06-24" @default.
• W155404871 creator A5005112936 @default.
• W155404871 date "2010-01-01" @default.
• W155404871 modified "2023-09-25" @default.
• W155404871 title "Spinning-Disk Confocal Microscopy of Yeast" @default.
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• W155404871 doi "https://doi.org/10.1016/s0076-6879(10)70023-9" @default.
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