Matches in SemOpenAlex for { <https://semopenalex.org/work/W1554150405> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1554150405 endingPage "343" @default.
- W1554150405 startingPage "335" @default.
- W1554150405 abstract "This chapter describes the purification of recombinant GIT1 and GIT2, and the assay of these proteins as ARF GAPs. The ARF family of GTP-binding proteins consists of six distinct ARF proteins, as well as several ARF-like (ARL) proteins and the ARF domain 1 (ARD1) protein. Individual recombinant ARF proteins are expressed in E. coli using a T7 RNA polymerase system and purified using ion exchange chromatography on DEAE-Sephacel and gel filtration chromatography using UltraGel AcA 54. The ability of GAPs to alter the guanine nucleotides bound to ARF proteins is assessed using a single-round GTP hydrolysis assay. ARF proteins are incubated with [α- 32 p]GTP to create the substrate for the GAP proteins (GTP-bound ARF) prior to addition of any GAP proteins." @default.
- W1554150405 created "2016-06-24" @default.
- W1554150405 creator A5027525105 @default.
- W1554150405 creator A5050538530 @default.
- W1554150405 date "2001-01-01" @default.
- W1554150405 modified "2023-09-27" @default.
- W1554150405 title "[36] Purification and characterization of GIT family of ADP-ribosylation factor (ARF) GTPase-activating proteins" @default.
- W1554150405 cites W1964024918 @default.
- W1554150405 cites W1976579206 @default.
- W1554150405 cites W1983657784 @default.
- W1554150405 cites W2008046589 @default.
- W1554150405 cites W2014295084 @default.
- W1554150405 cites W2030648310 @default.
- W1554150405 cites W2038454505 @default.
- W1554150405 cites W2081898060 @default.
- W1554150405 cites W2104353597 @default.
- W1554150405 cites W2121634203 @default.
- W1554150405 cites W2127943666 @default.
- W1554150405 cites W2140752038 @default.
- W1554150405 cites W2160033522 @default.
- W1554150405 cites W2231896361 @default.
- W1554150405 cites W4240922345 @default.
- W1554150405 cites W4245246789 @default.
- W1554150405 cites W4247868313 @default.
- W1554150405 doi "https://doi.org/10.1016/s0076-6879(01)29095-8" @default.
- W1554150405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11210553" @default.
- W1554150405 hasPublicationYear "2001" @default.
- W1554150405 type Work @default.
- W1554150405 sameAs 1554150405 @default.
- W1554150405 citedByCount "6" @default.
- W1554150405 countsByYear W15541504052013 @default.
- W1554150405 countsByYear W15541504052015 @default.
- W1554150405 countsByYear W15541504052017 @default.
- W1554150405 crossrefType "book-chapter" @default.
- W1554150405 hasAuthorship W1554150405A5027525105 @default.
- W1554150405 hasAuthorship W1554150405A5050538530 @default.
- W1554150405 hasConcept C119062480 @default.
- W1554150405 hasConcept C124682485 @default.
- W1554150405 hasConcept C158617107 @default.
- W1554150405 hasConcept C163181125 @default.
- W1554150405 hasConcept C181199279 @default.
- W1554150405 hasConcept C185592680 @default.
- W1554150405 hasConcept C207332259 @default.
- W1554150405 hasConcept C45510557 @default.
- W1554150405 hasConcept C55493867 @default.
- W1554150405 hasConcept C62478195 @default.
- W1554150405 hasConcept C75520062 @default.
- W1554150405 hasConcept C80631254 @default.
- W1554150405 hasConcept C86803240 @default.
- W1554150405 hasConcept C95444343 @default.
- W1554150405 hasConceptScore W1554150405C119062480 @default.
- W1554150405 hasConceptScore W1554150405C124682485 @default.
- W1554150405 hasConceptScore W1554150405C158617107 @default.
- W1554150405 hasConceptScore W1554150405C163181125 @default.
- W1554150405 hasConceptScore W1554150405C181199279 @default.
- W1554150405 hasConceptScore W1554150405C185592680 @default.
- W1554150405 hasConceptScore W1554150405C207332259 @default.
- W1554150405 hasConceptScore W1554150405C45510557 @default.
- W1554150405 hasConceptScore W1554150405C55493867 @default.
- W1554150405 hasConceptScore W1554150405C62478195 @default.
- W1554150405 hasConceptScore W1554150405C75520062 @default.
- W1554150405 hasConceptScore W1554150405C80631254 @default.
- W1554150405 hasConceptScore W1554150405C86803240 @default.
- W1554150405 hasConceptScore W1554150405C95444343 @default.
- W1554150405 hasLocation W15541504051 @default.
- W1554150405 hasLocation W15541504052 @default.
- W1554150405 hasOpenAccess W1554150405 @default.
- W1554150405 hasPrimaryLocation W15541504051 @default.
- W1554150405 hasRelatedWork W1549031993 @default.
- W1554150405 hasRelatedWork W1567005075 @default.
- W1554150405 hasRelatedWork W1971548708 @default.
- W1554150405 hasRelatedWork W2015701196 @default.
- W1554150405 hasRelatedWork W2027371255 @default.
- W1554150405 hasRelatedWork W2031579828 @default.
- W1554150405 hasRelatedWork W2103811827 @default.
- W1554150405 hasRelatedWork W2112905571 @default.
- W1554150405 hasRelatedWork W3016212309 @default.
- W1554150405 hasRelatedWork W3139296767 @default.
- W1554150405 isParatext "false" @default.
- W1554150405 isRetracted "false" @default.
- W1554150405 magId "1554150405" @default.
- W1554150405 workType "book-chapter" @default.