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• W1554274040 startingPage "958" @default.
• W1554274040 abstract "Bulk ribonucleic acid (RNA) was isolated from mechanically disrupted ascospores of Saccharomyces cerevisiae. After two passes over an oligo (dT10) cellulose column, the portion which bound, called poly(A)(+), was characterized. It is heterodisperse in size with a mean molecular weight of approximately 4 X 10(5), but contains some species as large as 7 X 10(5). The base composition is similar to vegetative poly(A)(+) RNA. The polyadenylate segment is also heterogenous in size, ranging from 90 to 20 bases in length, with a peak at approximately 60 nucleotides in length. Pulse-labeling of asci with [3H-methyl]methionine yields two caps, 7-methyl guanosine-5'-triphosphoryl-5'-adenosine (or guanosine) identical to that found in vegetative poly(A)(+) RNA. The poly(A)(+) RNA in spores is found in polyribosomes which are, on the average, smaller than vegetative ones. Long-term labeling studies indicate that the fraction of poly(A)(+) RNA in spores is similar to that in vegetative cells." @default.
• W1554274040 created "2016-06-24" @default.
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• W1554274040 date "1980-08-01" @default.
• W1554274040 modified "2023-09-25" @default.
• W1554274040 title "Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores" @default.
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• W1554274040 doi "https://doi.org/10.1128/jb.143.2.958-965.1980" @default.
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