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• W1554360427 abstract "Research Article1 February 1991free access A functional role for nucleosomes in the repression of a yeast promoter. C. Straka C. Straka Institut für Physiologische Chemie, Universität München, FRG. Search for more papers by this author W. Hörz W. Hörz Institut für Physiologische Chemie, Universität München, FRG. Search for more papers by this author C. Straka C. Straka Institut für Physiologische Chemie, Universität München, FRG. Search for more papers by this author W. Hörz W. Hörz Institut für Physiologische Chemie, Universität München, FRG. Search for more papers by this author Author Information C. Straka1 and W. Hörz1 1Institut für Physiologische Chemie, Universität München, FRG. The EMBO Journal (1991)10:361-368https://doi.org/10.1002/j.1460-2075.1991.tb07957.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Induction of the PHO5 gene in S. cerevisiae was previously shown to be accompanied by the removal of four positioned nucleosomes from the promoter. In order to assess the role of nucleosomes in the cascade of gene activation, DNA corresponding to one of these nucleosomes was excised. In its place two foreign DNA segments of the same length were inserted: a fragment from the African green monkey alpha-satellite DNA which is known to associate with histones in a highly specific fashion to give a uniquely positioned nucleosome or, alternatively, a fragment derived from pBR322 DNA. The promoter constructs were fused to the lacZ gene on centromere plasmids and transformed into yeast cells. The satellite fragment formed a nucleosome which persisted under inducing conditions. At the same time the inducibility of the PHO5 promoter was virtually abolished. When various subfragments containing between 35 and 100 bp of the satellite segment were tested, they were all found to decrease the inducibility of the promoter, full repression required the full length molecule, however. In contrast, the pBR fragment made the promoter weakly constitutive, and induction proceeded to levels even higher than with a promoter lacking an insert. Analysis of the chromatin structure reveals a nucleosome on the pBR segment at noninducing conditions which is removed upon induction. It is concluded that the quality of the histone-DNA interactions at the promoter makes an intrinsic contribution to the regulation of the gene. Previous ArticleNext Article Volume 10Issue 21 February 1991In this issue RelatedDetailsLoading ..." @default.
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• W1554360427 title "A functional role for nucleosomes in the repression of a yeast promoter." @default.
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• W1554360427 doi "https://doi.org/10.1002/j.1460-2075.1991.tb07957.x" @default.
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