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- W1554382128 abstract "PCBPl or poly (C) binding protein is a multifunctional protein, which belongs to the K homology domain superfamily. Its multiple functions include mRNA stabilization, translational regulation and transcriptional regulation, which have been found to affect the expression of the mu-opioid receptor gene. PCBPl's multiple functions may likely correlate to its various protein-protein interactions. We therefore examined its interacting protein(s) using a two-hybrid system by screening a cDNA library. After more than 1 million clones were screened, a candidate interacting protein was identified as ysynuclein. The plasmid of this cDNA clone was purified and the interaction between PCBPl and y-synuclein was further confirmed using in viva validation with the bacteria system as well as using GST-pull down assay. To further examine if this physical interaction can take place in a mammalian cell system, several cell lines, including NMB (neuroblastoma), H292 (lung), HCTl16 (colorectal), HT29 (colorectal), T47D (breast), and Mcf-7 (breast), were used. Western blot analysis showed that these human cancer cell lines endogenously expressed PCBP1 and ysynuclein. The physical interaction of these two proteins was examined using cell Iysates from these cell limes by co-Immunoprecipitation (co-IP) and subsequentially via Western blot analysis. Results suggested a physical interaction between endogenous PCBPl and y-synuclein. To further investigate if these cell lines expressed the identical 7-synuclein," @default.
- W1554382128 created "2016-06-24" @default.
- W1554382128 creator A5058961042 @default.
- W1554382128 date "2010-01-01" @default.
- W1554382128 modified "2023-09-27" @default.
- W1554382128 title "Investigation of the Protein-Protein Interaction Between PCBP1 and y -synuclein" @default.
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