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- W1554452023 abstract "Many proteins are modular in that they contain a series of independently-folded domains. Often, these sub-domains have no affinity for each other in solution, but can maintain a stable supertertiary structure when connected by polypeptide linkers. Little is known about the ultraweak interactions that dictate the stability of supertertiary structure. It is our hypothesis that surface features select for a specific structure, while the linker length determines the effective concentration thereby setting the interaction energy. To test this we attempted to redesign the multi-domain scaffold protein PSD-95. Structural studies have observed a stable supertertiary structure for the two N-terminal PDZ domains from PSD-95, but differing structural models were unable to identify interdomain interactions. We added a new interdomain salt bridge and linker extensions of varying rigidity. These manipulations rearranged the supertertiary structure but did not lead to isotropic motion of domains. This suggests that the forces stabilizing the structure are sufficient to withstand a 1.5-fold decrease in effective domain concentration. Grant Funding Source: Supported by NIMH Grant MH081923" @default.
- W1554452023 created "2016-06-24" @default.
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- W1554452023 date "2014-04-01" @default.
- W1554452023 modified "2023-10-18" @default.
- W1554452023 title "On the forces stabilizing supertertiary structure in multi‐domain proteins (770.2)" @default.
- W1554452023 doi "https://doi.org/10.1096/fasebj.28.1_supplement.770.2" @default.
- W1554452023 hasPublicationYear "2014" @default.
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