FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1554848618> ?p ?o ?g. }

• W1554848618 endingPage "4670" @default.
• W1554848618 startingPage "4663" @default.
• W1554848618 abstract "Abstract mAb Z22 is a highly selective IgG anti-Z-DNA Ab from an immunized C57BL/6 mouse. Previous studies showed that heavy chain CDR3 amino acids are critical for Z-DNA binding by the single chain variable fragment (scFv) comprising both V region heavy chain (VH) and V region light chain (VL) of mAb Z22 and that the VH domain alone binds Z-DNA with an affinity similar to that of whole variable fragment (Fv). To determine whether Z-DNA binding by VH alone and by Fv involves identical complementarity determining region residues, we tested effects of single or multiple amino acid substitutions in recombinant VH, scFv, and associated VH-VL heterodimers. Each recombinant product was a fusion protein with a B domain of Staphylococcal protein A (SPA). Z22VH-SPA alone was not highly selective; it bound strongly to other polynucleotides, particularly polypyrimidines, and ssDNA as well as to Z-DNA. In contrast, scFv-SPA or associated VH-VL dimers bound only to Z-DNA. VL-SPA domains bound weakly to Z-DNA; SPA alone did not bind. Introduction of multiple substitutions revealed that the third complementarity determining region of the heavy chain (CDR3H) was critical for both VH and scFv binding to Z-DNA. However, single substitutions that eliminated or markedly reduced Z-DNA binding by scFv instead caused a modest increase or no reduction in binding by VH alone. Association of VH-SPA with Z22VL-SPA restored both the effects of single substitutions and Z-DNA selectivity seen with Fv and intact Ab. Polypyrimidine and ssDNA binding by the isolated VH domain of immunization-induced anti-Z-DNA Ab resembles the activity of natural autoantibodies and suggests that VH-dependent binding to a ligand mimicked by polypyrimidines may play a role in B cell selection before immunization with Z-DNA." @default.
• W1554848618 created "2016-06-24" @default.
• W1554848618 creator A5037458498 @default.
• W1554848618 creator A5053689073 @default.
• W1554848618 date "1999-04-15" @default.
• W1554848618 modified "2023-10-11" @default.
• W1554848618 title "DNA Binding by the VH Domain of Anti-Z-DNA Antibody and Its Modulation by Association of the VL Domain" @default.
• W1554848618 cites W1484842414 @default.
• W1554848618 cites W1490657112 @default.
• W1554848618 cites W1496165222 @default.
• W1554848618 cites W1502845247 @default.
• W1554848618 cites W1508918383 @default.
• W1554848618 cites W1513332086 @default.
• W1554848618 cites W1520294975 @default.
• W1554848618 cites W1526921084 @default.
• W1554848618 cites W1547497180 @default.
• W1554848618 cites W1566581292 @default.
• W1554848618 cites W1567582032 @default.
• W1554848618 cites W1587793633 @default.
• W1554848618 cites W1605077028 @default.
• W1554848618 cites W1625145786 @default.
• W1554848618 cites W1654000141 @default.
• W1554848618 cites W1706098086 @default.
• W1554848618 cites W1968302870 @default.
• W1554848618 cites W1970757867 @default.
• W1554848618 cites W1981752145 @default.
• W1554848618 cites W1983824585 @default.
• W1554848618 cites W1991516961 @default.
• W1554848618 cites W2000228895 @default.
• W1554848618 cites W2002096606 @default.
• W1554848618 cites W2005997381 @default.
• W1554848618 cites W2010449143 @default.
• W1554848618 cites W2010664601 @default.
• W1554848618 cites W2013443854 @default.
• W1554848618 cites W2013882300 @default.
• W1554848618 cites W2019016721 @default.
• W1554848618 cites W2020972820 @default.
• W1554848618 cites W2022523419 @default.
• W1554848618 cites W2024702860 @default.
• W1554848618 cites W2031083473 @default.
• W1554848618 cites W2034271713 @default.
• W1554848618 cites W2035767894 @default.
• W1554848618 cites W2039969746 @default.
• W1554848618 cites W2047270330 @default.
• W1554848618 cites W2051729351 @default.
• W1554848618 cites W2052023333 @default.
• W1554848618 cites W2064729133 @default.
• W1554848618 cites W2066149280 @default.
• W1554848618 cites W2070123905 @default.
• W1554848618 cites W2081083186 @default.
• W1554848618 cites W2082046284 @default.
• W1554848618 cites W2088144279 @default.
• W1554848618 cites W2090383712 @default.
• W1554848618 cites W2095553811 @default.
• W1554848618 cites W2098285548 @default.
• W1554848618 cites W2113621372 @default.
• W1554848618 cites W2128092806 @default.
• W1554848618 cites W2134755638 @default.
• W1554848618 cites W2156086434 @default.
• W1554848618 cites W2160209304 @default.
• W1554848618 cites W2166317148 @default.
• W1554848618 cites W2272276626 @default.
• W1554848618 cites W4327650642 @default.
• W1554848618 doi "https://doi.org/10.4049/jimmunol.162.8.4663" @default.
• W1554848618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10202006" @default.
• W1554848618 hasPublicationYear "1999" @default.
• W1554848618 type Work @default.
• W1554848618 sameAs 1554848618 @default.
• W1554848618 citedByCount "13" @default.
• W1554848618 countsByYear W15548486182013 @default.
• W1554848618 countsByYear W15548486182016 @default.
• W1554848618 crossrefType "journal-article" @default.
• W1554848618 hasAuthorship W1554848618A5037458498 @default.
• W1554848618 hasAuthorship W1554848618A5053689073 @default.
• W1554848618 hasBestOaLocation W15548486181 @default.
• W1554848618 hasConcept C104317684 @default.
• W1554848618 hasConcept C111921641 @default.
• W1554848618 hasConcept C153911025 @default.
• W1554848618 hasConcept C159654299 @default.
• W1554848618 hasConcept C167625842 @default.
• W1554848618 hasConcept C185592680 @default.
• W1554848618 hasConcept C33987129 @default.
• W1554848618 hasConcept C36394416 @default.
• W1554848618 hasConcept C40767141 @default.
• W1554848618 hasConcept C54355233 @default.
• W1554848618 hasConcept C552990157 @default.
• W1554848618 hasConcept C55493867 @default.
• W1554848618 hasConcept C85630698 @default.
• W1554848618 hasConcept C86339819 @default.
• W1554848618 hasConcept C86803240 @default.
• W1554848618 hasConceptScore W1554848618C104317684 @default.
• W1554848618 hasConceptScore W1554848618C111921641 @default.
• W1554848618 hasConceptScore W1554848618C153911025 @default.
• W1554848618 hasConceptScore W1554848618C159654299 @default.
• W1554848618 hasConceptScore W1554848618C167625842 @default.
• W1554848618 hasConceptScore W1554848618C185592680 @default.
• W1554848618 hasConceptScore W1554848618C33987129 @default.
• W1554848618 hasConceptScore W1554848618C36394416 @default.

• next