Matches in SemOpenAlex for { <https://semopenalex.org/work/W1554897462> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1554897462 endingPage "26958" @default.
- W1554897462 startingPage "26953" @default.
- W1554897462 abstract "The D-glucuronyl C-5 epimerase involved in the biosynthesis of heparin/heparan sulfate was purified from the high speed supernatant fraction of a homogenate of bovine liver by chromatography on immobilized O-desulfated heparin, red Sepharose, phenyl Sepharose, and concanavalin A-Sepharose. After close to 1 million-fold purification, in 10-15% yield, the product gave a single band on SDS-polyacrylamide gel electrophoresis with silver staining and had a mobility corresponding to an M(r) of approximately 52,000. Since the epimerase assay used in the course of purification was based on release of tritium, as [3H]H2O, from a [5-3H]uronyl-labeled substrate, it was important to establish that the purified enzyme did indeed catalyze the actual conversion of D-glucuronyl to L-iduronyl residues. Upon incubation of the purified enzyme with 3H-labeled heparosan N-sulfate, prepared by metabolic labeling (with D-[1-3H]glucose) of a capsular polysaccharide from Escherichia coli K5 and subsequent chemical partial N-deacetylation and N-sulfation, approximately 30% of the D-glucuronyl residues located between two N-sulfated glucosamine units were converted to L-iduronyl units." @default.
- W1554897462 created "2016-06-24" @default.
- W1554897462 creator A5001998362 @default.
- W1554897462 creator A5011211993 @default.
- W1554897462 creator A5047169163 @default.
- W1554897462 creator A5058957230 @default.
- W1554897462 creator A5071436681 @default.
- W1554897462 creator A5088174305 @default.
- W1554897462 date "1994-10-01" @default.
- W1554897462 modified "2023-10-16" @default.
- W1554897462 title "Biosynthesis of heparin/heparan sulfate. Purification of the D-glucuronyl C-5 epimerase from bovine liver." @default.
- W1554897462 cites W143705426 @default.
- W1554897462 cites W1540551989 @default.
- W1554897462 cites W1544749959 @default.
- W1554897462 cites W1576755772 @default.
- W1554897462 cites W1587239867 @default.
- W1554897462 cites W1623765232 @default.
- W1554897462 cites W1878854037 @default.
- W1554897462 cites W2005681212 @default.
- W1554897462 cites W2011403137 @default.
- W1554897462 cites W2014321075 @default.
- W1554897462 cites W2020731397 @default.
- W1554897462 cites W2033957404 @default.
- W1554897462 cites W2040427415 @default.
- W1554897462 cites W2041420763 @default.
- W1554897462 cites W2065628860 @default.
- W1554897462 cites W2100837269 @default.
- W1554897462 cites W2328011468 @default.
- W1554897462 cites W3173300771 @default.
- W1554897462 cites W4247585738 @default.
- W1554897462 cites W4293247451 @default.
- W1554897462 doi "https://doi.org/10.1016/s0021-9258(18)47111-6" @default.
- W1554897462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7929434" @default.
- W1554897462 hasPublicationYear "1994" @default.
- W1554897462 type Work @default.
- W1554897462 sameAs 1554897462 @default.
- W1554897462 citedByCount "40" @default.
- W1554897462 countsByYear W15548974622012 @default.
- W1554897462 countsByYear W15548974622013 @default.
- W1554897462 countsByYear W15548974622014 @default.
- W1554897462 countsByYear W15548974622015 @default.
- W1554897462 countsByYear W15548974622019 @default.
- W1554897462 countsByYear W15548974622020 @default.
- W1554897462 countsByYear W15548974622021 @default.
- W1554897462 crossrefType "journal-article" @default.
- W1554897462 hasAuthorship W1554897462A5001998362 @default.
- W1554897462 hasAuthorship W1554897462A5011211993 @default.
- W1554897462 hasAuthorship W1554897462A5047169163 @default.
- W1554897462 hasAuthorship W1554897462A5058957230 @default.
- W1554897462 hasAuthorship W1554897462A5071436681 @default.
- W1554897462 hasAuthorship W1554897462A5088174305 @default.
- W1554897462 hasBestOaLocation W15548974621 @default.
- W1554897462 hasConcept C153074725 @default.
- W1554897462 hasConcept C178790620 @default.
- W1554897462 hasConcept C181199279 @default.
- W1554897462 hasConcept C185592680 @default.
- W1554897462 hasConcept C2777557582 @default.
- W1554897462 hasConcept C2778041096 @default.
- W1554897462 hasConcept C2778343803 @default.
- W1554897462 hasConcept C553450214 @default.
- W1554897462 hasConcept C55493867 @default.
- W1554897462 hasConceptScore W1554897462C153074725 @default.
- W1554897462 hasConceptScore W1554897462C178790620 @default.
- W1554897462 hasConceptScore W1554897462C181199279 @default.
- W1554897462 hasConceptScore W1554897462C185592680 @default.
- W1554897462 hasConceptScore W1554897462C2777557582 @default.
- W1554897462 hasConceptScore W1554897462C2778041096 @default.
- W1554897462 hasConceptScore W1554897462C2778343803 @default.
- W1554897462 hasConceptScore W1554897462C553450214 @default.
- W1554897462 hasConceptScore W1554897462C55493867 @default.
- W1554897462 hasIssue "43" @default.
- W1554897462 hasLocation W15548974621 @default.
- W1554897462 hasOpenAccess W1554897462 @default.
- W1554897462 hasPrimaryLocation W15548974621 @default.
- W1554897462 hasRelatedWork W1588494769 @default.
- W1554897462 hasRelatedWork W1978799551 @default.
- W1554897462 hasRelatedWork W1995003331 @default.
- W1554897462 hasRelatedWork W2019185576 @default.
- W1554897462 hasRelatedWork W2027074537 @default.
- W1554897462 hasRelatedWork W2039276558 @default.
- W1554897462 hasRelatedWork W2054965394 @default.
- W1554897462 hasRelatedWork W2108350011 @default.
- W1554897462 hasRelatedWork W2163616680 @default.
- W1554897462 hasRelatedWork W2328912603 @default.
- W1554897462 hasVolume "269" @default.
- W1554897462 isParatext "false" @default.
- W1554897462 isRetracted "false" @default.
- W1554897462 magId "1554897462" @default.
- W1554897462 workType "article" @default.