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- W1555094217 abstract "Human African sleeping sickness (HAT) is caused by the parasitic protozoan Trypanosoma brucei. Polyamine biosynthesis is an important drug target in the treatment of HAT. Previously we showed that trypanosomatid S-adenosylmethionine decarboxylase (AdoMetDC), a key enzyme for biosynthesis of the polyamine spermidine, is activated by heterodimer formation with an inactive paralogue termed prozyme. Furthermore, prozyme protein levels were regulated in response to reduced AdoMetDC activity. Herein we show that T. brucei encodes three prozyme transcripts. The 3′UTRs of these transcripts were mapped and chloramphenicol acetyltransferase (CAT) reporter constructs were used to identify a 1.2 kb region that contained a 3′UTR prozyme regulatory element sufficient to upregulate CAT protein levels (but not RNA) upon AdoMetDC inhibition, supporting the hypothesis that prozyme expression is regulated translationally. To gain insight into trans-acting factors, genetic rescue of AdoMetDC RNAi knock-down lines with human AdoMetDC was performed leading to rescue of the cell growth block, and restoration of prozyme protein to wild-type levels. Metabolite analysis showed that prozyme protein levels were inversely proportional to intracellular levels of decarboxylated AdoMet (dcAdoMet). These data suggest that prozyme translation may be regulated by dcAdoMet, a metabolite not previously identified to play a regulatory role." @default.
- W1555094217 created "2016-06-24" @default.
- W1555094217 creator A5008835508 @default.
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- W1555094217 creator A5026350486 @default.
- W1555094217 creator A5044347237 @default.
- W1555094217 creator A5055921315 @default.
- W1555094217 date "2013-05-02" @default.
- W1555094217 modified "2023-09-26" @default.
- W1555094217 title "Product feedback regulation implicated in translational control of the<i>Trypanosoma brucei S</i>-adenosylmethionine decarboxylase regulatory subunit prozyme" @default.
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- W1555094217 doi "https://doi.org/10.1111/mmi.12226" @default.
- W1555094217 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3771639" @default.
- W1555094217 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23634831" @default.
- W1555094217 hasPublicationYear "2013" @default.
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