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• W1555149048 abstract "ANALISE HEMATOLOGICA E BIOQUIMICA COMPARATIVA EM SUINOS SUBMETIDOS A OBSTRUCAO BILIAR EXPERIMENTAL RESUMO Este trabalho objetivou avaliar as alteracoes hematologicas e bioquimicas decorrentes de obstrucao biliar induzida em suinos. Para isso, foram utilizados seis suinos da raca Landrace, com 36 dias de idade, nos quais coletou-se sangue nos momentos pre-obstrucao e sete dias apos a obstrucao induzida do ducto coledoco, sendo a mesma realizada pela colocacao videolaparoscopica de um clip metalico de titânio. Assim, a ligadura foi realizada com sucesso em todos os animais, sendo facil a identificacao do coledoco por videolaparoscopia e nao ocorrendo dificuldades nos procedimentos. Os sinais clinicos indicativos de ictericia foram evidentes no decorrer dos sete dias de obstrucao. A analise hematologica revelou uma diminuicao nos niveis do volume corpuscular medio e permitiu identificar alteracoes na morfologia das hemacias; enquanto que os exames bioquimicos revelaram aumento nos niveis sericos de colesterol, GGT, FA, bilirrubina total e fracoes e, reducao na glicemia. Conclui-se que o suino representa um bom modelo experimental de obstrucao biliar, tendo como caracteristicas evidentes a hiperbilirrubinuria e o aumento dos niveis normais das enzimas hepatobiliares. A GGT demonstrou-se um marcador de maior sensibilidade e especificidade em suinos quando comparada com a FA. Palavras-chave: contagem de celulas sanguineas, bioquimica, ictericia obstrutiva, colestase extra-hepatica, laparoscopia HEMATOLOGY AND BIOCHEMISTRY COMPARATIVE ANALYSIS IN SWINES SUBMITTED TO EXPERIMENTAL BILIARY OBSTRUCTION ABSTRACT This study aimed to evaluate hematological and biochemical changes resulting from biliary obstruction induced in swines. For this, were used six Landrace swines of 36-day-old, blood were collected in the pre-obstruction and seven days after the induced common bile duct obstruction, which was done through the laparoscopic placement of a titanium metal clip. Thus, the ligation was successfully performed in all animals, being easier to identify the laparoscopic common bile duct and not occurring difficult procedures. The clinical signs of jaundice were evident after seven days of obstruction. The blood analysis revealed a decrease in the levels of mean corpuscular volume and identified changes in the morphology of red blood cells, whereas the biochemical tests revealed increased serum levels of cholesterol, gamma-glutamyltransferase, alkaline phosphatase, total bilirubin and fractions and, reduction in blood glucose. It is concluded that the pig is a good experimental model of biliary obstruction, as having the characteristics evident hiperbilirrubinuria and increased normal levels of hepatobiliary enzymes. The gamma-glutamyl transferase proved to be a marker of greater sensitivity and specificity in pigs when compared with the alkaline phosphatase. Keywords : blood cell count, biochemistry, jaundice obstructive, cholestasis extrahepatic, laparoscopy ANALISIS HEMATOLOGICO Y BIOQUIMICO COMPARATIVO EN CERDOS SOMETIDOS A OBSTRUCCION BILIAR EXPERIMENTAL RESUMEN Este estudio tuvo como objetivo evaluar las alteraciones hematologicas y bioquimicas causadas por la obstruccion biliar inducida en cerdos. Para ello, se utilizaron seis cerdos Landrace, con 36 dias de edad. Fueron obtenidas muestras sanguineas antes y siete dias despues de la obstruccion inducida del conducto biliar, la misma que se logro con la colocacion de un clip metalico de titanio a traves de laparoscopia. La ligadura se llevo a cabo con exito en todos los animales. El conducto biliar comun fue facilmente identificado en la videolaparoscopia y no hubo dificultades para la realizacion del procedimiento. Signos clinicos como la ictericia fueron evidentes durante los siete dias de la obstruccion. El analisis de sangre revelo una disminucion en el volumen corpuscular medio y cambios en la morfologia de los globulos rojos, mientras que las pruebas bioquimicas indicaron aumento de los niveles sericos de colesterol, gammaglutamil transferasa, fosfatasa alcalina, bilirrubina total y sus fracciones, y reduccion de la glucosa en la sangre. Se concluye que el cerdo es un buen modelo experimental de obstruccion biliar, con caracteristicas evidentes como hiperbilirrubinuria y aumento de los niveles normales de enzimas hepatobiliares. La gammaglutamil transferasa demostro ser un marcador de mayor sensibilidad y especificidad en los cerdos, en comparacion con la fosfatasa alcalina. Palabras-clave: contaje de celulas sanguineas, bioquimica, ictericia obstructiva, colestasis extra-hepatica, laparoscopia" @default.
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• W1555149048 date "2013-12-01" @default.
• W1555149048 modified "2023-10-17" @default.
• W1555149048 title "ANÁLISE HEMATOLÓGICA E BIOQUÍMICA COMPARATIVA EM SUÍNOS SUBMETIDOS À OBSTRUÇÃO BILIAR EXPERIMENTAL" @default.
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