Matches in SemOpenAlex for { <https://semopenalex.org/work/W1555367722> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1555367722 endingPage "2079" @default.
- W1555367722 startingPage "2079" @default.
- W1555367722 abstract "Investigation of Pneumocystis jirovecii colonization in patients with chronic pulmonary diseases in the People’s Republic of China Dong-Dong Wang,1 Ming-Quan Zheng,2,3 Nan Zhang,2 Chun-Li An2 1Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, People’s Republic of China; 2Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang, People’s Republic of China; 3Richard King Mellon Foundation Institute for Pediatric Research, Children’s Hospital of Pittsburgh of UPMC, Pittsburgh, PA, USABackground: The detection of Pneumocystis jirovecii DNA in respiratory specimen from individuals who do not have signs or symptoms of pneumonia has been defined as colonization. The role of P. jirovecii colonization in the development or progression of various lung diseases has been reported, but little information about P. jirovecii colonization in patients is available in the People’s Republic of China.Objective: To determine the prevalence of P. jirovecii colonization in patients with various pulmonary diseases, including the acute and stable stage of COPD, interstitial lung diseases, cystic fibrosis, and chronic bronchiectasis.Materials and methods: A loop-mediated isothermal amplification (LAMP) and a conventional polymerase chain reaction (PCR) method for detecting P. jirovecii were developed. Ninety-eight HIV-negative patients who were followed-up and who had undergone bronchoscopy for diagnosis of various underlying respiratory diseases were included in the study. Sputa of these patients were analyzed with LAMP amplification of P. jirovecii gene. In addition, conventional PCR, Giemsa and Gomori’s methenamine silver nitrate staining assays were applied to all specimens.Results: The sensitivity and specificity test showed that there was no cross-reaction with other fungi or bacteria in detecting the specific gene of P. jirovecii by LAMP, and the minimum detection limits by LAMP was 50 copies/mL. P. jirovecii DNA was detected in 62 of 98 (63.3%) sputa specimens by LAMP assay and 22.45% (22/98) by conventional PCR. However, no P. jirovecii cysts were found by Giemsa and Gomori’s methenamine silver nitrate in all of gene-positive specimens.Conclusion: The results of our study showed that prevalence of P. jirovecii colonization is particularly high in patients with chronic pulmonary diseases in People’s Republic of China, and the LAMP method is better for evaluation of the colonization of P. jirovecii in sputum specimen than conventional PCR. Keywords: Pneumocystis jirovecii, colonization, chronic pulmonary diseases, loop-mediated isothermal amplification" @default.
- W1555367722 created "2016-06-24" @default.
- W1555367722 creator A5009318195 @default.
- W1555367722 creator A5042571447 @default.
- W1555367722 creator A5086523387 @default.
- W1555367722 creator A5087297830 @default.
- W1555367722 date "2015-09-01" @default.
- W1555367722 modified "2023-10-16" @default.
- W1555367722 title "Investigation of Pneumocystis jirovecii colonization in patients with chronic pulmonary diseases in the People’s Republic of China" @default.
- W1555367722 cites W1571239990 @default.
- W1555367722 cites W1978783962 @default.
- W1555367722 cites W1981865539 @default.
- W1555367722 cites W1986747413 @default.
- W1555367722 cites W1987141965 @default.
- W1555367722 cites W1997970727 @default.
- W1555367722 cites W1999547061 @default.
- W1555367722 cites W2058577835 @default.
- W1555367722 cites W2086026028 @default.
- W1555367722 cites W2096591112 @default.
- W1555367722 cites W2098515639 @default.
- W1555367722 cites W2104518947 @default.
- W1555367722 cites W2113047013 @default.
- W1555367722 cites W2114865585 @default.
- W1555367722 cites W2125554916 @default.
- W1555367722 cites W2126964298 @default.
- W1555367722 cites W2137089903 @default.
- W1555367722 cites W2142039600 @default.
- W1555367722 cites W2145845672 @default.
- W1555367722 cites W2155954788 @default.
- W1555367722 cites W2164805437 @default.
- W1555367722 cites W2164830901 @default.
- W1555367722 cites W2172064173 @default.
- W1555367722 cites W2334286687 @default.
- W1555367722 cites W900876721 @default.
- W1555367722 doi "https://doi.org/10.2147/copd.s89666" @default.
- W1555367722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4598221" @default.
- W1555367722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26491278" @default.
- W1555367722 hasPublicationYear "2015" @default.
- W1555367722 type Work @default.
- W1555367722 sameAs 1555367722 @default.
- W1555367722 citedByCount "12" @default.
- W1555367722 countsByYear W15553677222017 @default.
- W1555367722 countsByYear W15553677222018 @default.
- W1555367722 countsByYear W15553677222019 @default.
- W1555367722 countsByYear W15553677222020 @default.
- W1555367722 countsByYear W15553677222021 @default.
- W1555367722 countsByYear W15553677222022 @default.
- W1555367722 countsByYear W15553677222023 @default.
- W1555367722 crossrefType "journal-article" @default.
- W1555367722 hasAuthorship W1555367722A5009318195 @default.
- W1555367722 hasAuthorship W1555367722A5042571447 @default.
- W1555367722 hasAuthorship W1555367722A5086523387 @default.
- W1555367722 hasAuthorship W1555367722A5087297830 @default.
- W1555367722 hasBestOaLocation W15553677221 @default.
- W1555367722 hasConcept C126322002 @default.
- W1555367722 hasConcept C142724271 @default.
- W1555367722 hasConcept C190139176 @default.
- W1555367722 hasConcept C190612196 @default.
- W1555367722 hasConcept C203014093 @default.
- W1555367722 hasConcept C2777559004 @default.
- W1555367722 hasConcept C2777914695 @default.
- W1555367722 hasConcept C71924100 @default.
- W1555367722 hasConceptScore W1555367722C126322002 @default.
- W1555367722 hasConceptScore W1555367722C142724271 @default.
- W1555367722 hasConceptScore W1555367722C190139176 @default.
- W1555367722 hasConceptScore W1555367722C190612196 @default.
- W1555367722 hasConceptScore W1555367722C203014093 @default.
- W1555367722 hasConceptScore W1555367722C2777559004 @default.
- W1555367722 hasConceptScore W1555367722C2777914695 @default.
- W1555367722 hasConceptScore W1555367722C71924100 @default.
- W1555367722 hasLocation W15553677221 @default.
- W1555367722 hasLocation W15553677222 @default.
- W1555367722 hasLocation W15553677223 @default.
- W1555367722 hasLocation W15553677224 @default.
- W1555367722 hasOpenAccess W1555367722 @default.
- W1555367722 hasPrimaryLocation W15553677221 @default.
- W1555367722 hasRelatedWork W1877883789 @default.
- W1555367722 hasRelatedWork W1994948255 @default.
- W1555367722 hasRelatedWork W1995384656 @default.
- W1555367722 hasRelatedWork W2024711449 @default.
- W1555367722 hasRelatedWork W2025472954 @default.
- W1555367722 hasRelatedWork W2029147028 @default.
- W1555367722 hasRelatedWork W2043775871 @default.
- W1555367722 hasRelatedWork W2077480214 @default.
- W1555367722 hasRelatedWork W2167116812 @default.
- W1555367722 hasRelatedWork W2316236384 @default.
- W1555367722 isParatext "false" @default.
- W1555367722 isRetracted "false" @default.
- W1555367722 magId "1555367722" @default.
- W1555367722 workType "article" @default.