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• W1555458718 abstract "[35S]S-[5-(4-benzoylphenyl)pentyl]glutathione (GIF-0017) as a biochemical probe targeting the ATP-dependent organic anion transporters GS-X pumps was synthesized by the reaction of [35S]glutathione and excess 4-(5-bromo)pentylbenzophenone under alkaline conditions, with the radiochemical yield of 24-33% after HPLC purification. Photolysis of the mixture of [35S]GIF-0017 and plasma membrane vesicles prepared from the MRP1 cDNA-transfected LLC-PK1 cells resulted in radio-labeling of a 180-kDa membrane protein. Immunoprecipitation and western blotting using an anti-MRP1 monoclonal antibody confirmed that the [35S]GIF-0017-labeled protein was the MRPI/GS-X pump." @default.
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• W1555458718 date "1999-09-01" @default.
• W1555458718 modified "2023-09-27" @default.
• W1555458718 title ": A versatile radioligand targeting GS-X pumps with the ability of photoaffinity labeling" @default.
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• W1555458718 doi "https://doi.org/10.1016/s0960-894x(99)00455-2" @default.
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