Matches in SemOpenAlex for { <https://semopenalex.org/work/W1555884348> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1555884348 endingPage "17726" @default.
- W1555884348 startingPage "17722" @default.
- W1555884348 abstract "In a highly ouabain-resistant clone from the Madin-Darby canine kidney cell line (Ki > 4 mM), we have previously identified mutations (C113 to Y or C113 to F) in the first transmembrane helix (H1) of the Na,K-ATPase alpha subunit that increase the Ki of a ouabain-sensitive Na,K pump by 1000-fold. Here we report another mutation (Y317 to C) located in the extracellular segment that joins the third and fourth transmembrane domains (H3-H4 ectodomain) of the alpha subunit that also changes ouabain sensitivity of the Na pump. When this mutation (Y317C) was introduced into the Na,K-ATPase alpha 1 subunit of Xenopus laevis, the ouabain inhibition constant increased by a factor of 5, from 130 (wild type) to 800 nM (mutant). However, the expression of double mutants (C113Y + Y317C) in Xenopus oocytes resulted in highly ouabain-resistant Na,K pumps (Ki approximately 7 mM), reproducing the phenotype of the original Madin-Darby canine kidney cell line. When a more conservative change (Y317F) was introduced into the Na,K-ATPase alpha 1 subunit of X. laevis, the ouabain koff increased and expression of double mutants (C113Y + Y317F) resulted in an intermediate ouabain-resistant Na,K pump (Ki approximately 500 microM). We propose that, in addition to the previously identified H1-H2 ectodomain of Na,K-ATPase alpha subunit, the H3-H4 ectodomain also participates in the structure and/or the function of the ouabain binding site. In this respect, the Y317 plays a critical role since the most conservative change Y313 to F is sufficient to significantly affect ouabain binding." @default.
- W1555884348 created "2016-06-24" @default.
- W1555884348 creator A5011607696 @default.
- W1555884348 creator A5040501423 @default.
- W1555884348 creator A5061532717 @default.
- W1555884348 date "1993-08-01" @default.
- W1555884348 modified "2023-09-29" @default.
- W1555884348 title "Mutation of a tyrosine in the H3-H4 ectodomain of Na,K-ATPase alpha subunit confers ouabain resistance." @default.
- W1555884348 cites W1516345257 @default.
- W1555884348 cites W1516407458 @default.
- W1555884348 cites W1517936476 @default.
- W1555884348 cites W1536844713 @default.
- W1555884348 cites W1550982765 @default.
- W1555884348 cites W1584531078 @default.
- W1555884348 cites W1967604793 @default.
- W1555884348 cites W1992098567 @default.
- W1555884348 cites W2011242077 @default.
- W1555884348 cites W2022599332 @default.
- W1555884348 cites W2093166155 @default.
- W1555884348 cites W2163939900 @default.
- W1555884348 cites W963952221 @default.
- W1555884348 doi "https://doi.org/10.1016/s0021-9258(17)46764-0" @default.
- W1555884348 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8394348" @default.
- W1555884348 hasPublicationYear "1993" @default.
- W1555884348 type Work @default.
- W1555884348 sameAs 1555884348 @default.
- W1555884348 citedByCount "47" @default.
- W1555884348 countsByYear W15558843482012 @default.
- W1555884348 countsByYear W15558843482015 @default.
- W1555884348 countsByYear W15558843482016 @default.
- W1555884348 crossrefType "journal-article" @default.
- W1555884348 hasAuthorship W1555884348A5011607696 @default.
- W1555884348 hasAuthorship W1555884348A5040501423 @default.
- W1555884348 hasAuthorship W1555884348A5061532717 @default.
- W1555884348 hasBestOaLocation W15558843481 @default.
- W1555884348 hasConcept C1009742 @default.
- W1555884348 hasConcept C104292427 @default.
- W1555884348 hasConcept C104317684 @default.
- W1555884348 hasConcept C153911025 @default.
- W1555884348 hasConcept C159110408 @default.
- W1555884348 hasConcept C170493617 @default.
- W1555884348 hasConcept C178790620 @default.
- W1555884348 hasConcept C185592680 @default.
- W1555884348 hasConcept C2775944032 @default.
- W1555884348 hasConcept C2776165026 @default.
- W1555884348 hasConcept C2779133857 @default.
- W1555884348 hasConcept C49453240 @default.
- W1555884348 hasConcept C501734568 @default.
- W1555884348 hasConcept C537181965 @default.
- W1555884348 hasConcept C55493867 @default.
- W1555884348 hasConcept C64943373 @default.
- W1555884348 hasConcept C71924100 @default.
- W1555884348 hasConcept C83730767 @default.
- W1555884348 hasConcept C86803240 @default.
- W1555884348 hasConceptScore W1555884348C1009742 @default.
- W1555884348 hasConceptScore W1555884348C104292427 @default.
- W1555884348 hasConceptScore W1555884348C104317684 @default.
- W1555884348 hasConceptScore W1555884348C153911025 @default.
- W1555884348 hasConceptScore W1555884348C159110408 @default.
- W1555884348 hasConceptScore W1555884348C170493617 @default.
- W1555884348 hasConceptScore W1555884348C178790620 @default.
- W1555884348 hasConceptScore W1555884348C185592680 @default.
- W1555884348 hasConceptScore W1555884348C2775944032 @default.
- W1555884348 hasConceptScore W1555884348C2776165026 @default.
- W1555884348 hasConceptScore W1555884348C2779133857 @default.
- W1555884348 hasConceptScore W1555884348C49453240 @default.
- W1555884348 hasConceptScore W1555884348C501734568 @default.
- W1555884348 hasConceptScore W1555884348C537181965 @default.
- W1555884348 hasConceptScore W1555884348C55493867 @default.
- W1555884348 hasConceptScore W1555884348C64943373 @default.
- W1555884348 hasConceptScore W1555884348C71924100 @default.
- W1555884348 hasConceptScore W1555884348C83730767 @default.
- W1555884348 hasConceptScore W1555884348C86803240 @default.
- W1555884348 hasIssue "24" @default.
- W1555884348 hasLocation W15558843481 @default.
- W1555884348 hasOpenAccess W1555884348 @default.
- W1555884348 hasPrimaryLocation W15558843481 @default.
- W1555884348 hasRelatedWork W1510227168 @default.
- W1555884348 hasRelatedWork W1555884348 @default.
- W1555884348 hasRelatedWork W1575835136 @default.
- W1555884348 hasRelatedWork W1580948719 @default.
- W1555884348 hasRelatedWork W1590324536 @default.
- W1555884348 hasRelatedWork W1590783189 @default.
- W1555884348 hasRelatedWork W1878835465 @default.
- W1555884348 hasRelatedWork W2013725032 @default.
- W1555884348 hasRelatedWork W2408934738 @default.
- W1555884348 hasRelatedWork W2535251566 @default.
- W1555884348 hasVolume "268" @default.
- W1555884348 isParatext "false" @default.
- W1555884348 isRetracted "false" @default.
- W1555884348 magId "1555884348" @default.
- W1555884348 workType "article" @default.