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• W155647511 abstract "The studies presented in this thesis contribute to improved resolution of genetic variability and diagnosis of potyviruses co-infecting sweetpotatoes. The most prevalent viruses infecting sweetpotato in Tanzania were Sweet potato feathery mottle virus (SPFMV), Sweet potato mild mottle virus (SPMMV), Sweet potato chlorotic stunt virus (SPCSV) and Sweet potato chlorotic fleck virus (SPCFV). They were serologically detected and results confirmed by RT-PCR and sequencing. Nucleotide (nt) sequences of the 3´-proximal genomic part of SPFMV and SPMMV and partial HSP70h gene of SPCSV isolates from Tanzania and Uganda were determined. Results revealed limited genetic variability among East African (EA) isolates for SPFMV and SPCSV but high variability for SPMMV. Phylogenetic analysis grouped Tanzanian and Ugandan isolates into an EA strain for SPFMV and SPCSV. Phylogenetically, four strains of SPFMV were detected: EA, RC and O, and strain C which is distantly related to them. SPCSV isolates were grouped into two distantly related strains: EA group with only East African isolates, and a non-East African (NEA) group with isolates from West Africa and other parts of the world. No distinct strain groups could be inferred among the SPMMV isolates from East Africa In Australia, Sweet potato virus Y (SPVY; synonymous to SPV-2) (family Potyviridae) was found to co-infect sweetpotatoes with strains RC and/or C of SPFMV. Five SPVY and SPFMV isolates were sequence-characterised for their 3’-proximal end of the genome and compared with isolates from Asia, Africa, Europe and North America. Phylogenetic analysis showed that Australian SPVY isolates formed a separate clade closest to the North American isolates. Utilising findings of molecular resolutions of major viruses in East Africa and Australia, a simple and sensitive RT-PCR/RFLP based diagnostic procedure was devised to differentiate potyviruses in complexes which readily differentiated SPVY and strains C and RC of SPFMV." @default.
• W155647511 created "2016-06-24" @default.
• W155647511 creator A5063733113 @default.
• W155647511 date "2006-02-01" @default.
• W155647511 modified "2023-09-27" @default.
• W155647511 title "Molecular Resolution of Genetic Variability of Major Sweetpotato Viruses and Improved Diagnosis of Potyviruses Co-infecting Sweetpotato" @default.
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