Matches in SemOpenAlex for { <https://semopenalex.org/work/W1556482919> ?p ?o ?g. }
Showing items 1 to 54 of
54
with 100 items per page.
- W1556482919 endingPage "53" @default.
- W1556482919 startingPage "53" @default.
- W1556482919 abstract "The application of conditionally replicating adenoviruses to cancer gene therapy has shown much promise. However, it has become apparent that these vectors must be more specifically re-targeted to increase therapeutic effect. Although the number of re-targeted vectors is growing, the total number is small, mostly due to cloning difficulties associated with the large viral genome. Here, we have developed a unidirectional shuttle exchange system, called pFex, where one can easily transfer modified fiber genes from a smaller shuttle vector directly into the fiber portion of the Adenovirus genome. The adenoviral acceptor vector, termed pFex, was derived from the AdEasy1 vector where the entire fiber gene has been replaced by the sucrose lethal gene, SacB, and two flanking non-compatible half-mutant lox sites. Several smaller shuttle vectors contain the modified fiber gene, which is also flanked by two non-compatible half mutant lox sites. In the presence of Cre recombinase, the modified fiber gene is shuttled into pFex, replacing SacB. The resulting lox sites are fully mutated and no longer recognized by Cre recombinase, producing a unidirectional exchange. The combination of several selection markers allows one to exchange fiber either before or after the traditional RecA mediated E1 region recombination. The pFex system is highly efficient and has been used to produce eight recombinant viral vectors in less than two weeks. This exchange system should dramatically increase the output of re-targeting adenoviral vectors." @default.
- W1556482919 created "2016-06-24" @default.
- W1556482919 creator A5015177135 @default.
- W1556482919 creator A5081198528 @default.
- W1556482919 creator A5088504106 @default.
- W1556482919 date "2004-05-01" @default.
- W1556482919 modified "2023-09-23" @default.
- W1556482919 title "pFex: a novel fiber exchange system for the creation of modified-fiber gene therapy vectors" @default.
- W1556482919 doi "https://doi.org/10.1016/j.ymthe.2004.06.093" @default.
- W1556482919 hasPublicationYear "2004" @default.
- W1556482919 type Work @default.
- W1556482919 sameAs 1556482919 @default.
- W1556482919 citedByCount "0" @default.
- W1556482919 crossrefType "journal-article" @default.
- W1556482919 hasAuthorship W1556482919A5015177135 @default.
- W1556482919 hasAuthorship W1556482919A5081198528 @default.
- W1556482919 hasAuthorship W1556482919A5088504106 @default.
- W1556482919 hasBestOaLocation W15564829191 @default.
- W1556482919 hasConcept C104317684 @default.
- W1556482919 hasConcept C111599444 @default.
- W1556482919 hasConcept C159985019 @default.
- W1556482919 hasConcept C192562407 @default.
- W1556482919 hasConcept C519885992 @default.
- W1556482919 hasConcept C54355233 @default.
- W1556482919 hasConcept C70721500 @default.
- W1556482919 hasConcept C86803240 @default.
- W1556482919 hasConceptScore W1556482919C104317684 @default.
- W1556482919 hasConceptScore W1556482919C111599444 @default.
- W1556482919 hasConceptScore W1556482919C159985019 @default.
- W1556482919 hasConceptScore W1556482919C192562407 @default.
- W1556482919 hasConceptScore W1556482919C519885992 @default.
- W1556482919 hasConceptScore W1556482919C54355233 @default.
- W1556482919 hasConceptScore W1556482919C70721500 @default.
- W1556482919 hasConceptScore W1556482919C86803240 @default.
- W1556482919 hasLocation W15564829191 @default.
- W1556482919 hasOpenAccess W1556482919 @default.
- W1556482919 hasPrimaryLocation W15564829191 @default.
- W1556482919 hasRelatedWork W1603543036 @default.
- W1556482919 hasRelatedWork W2000695783 @default.
- W1556482919 hasRelatedWork W2363972173 @default.
- W1556482919 hasRelatedWork W2375619838 @default.
- W1556482919 hasRelatedWork W2621242303 @default.
- W1556482919 hasRelatedWork W2837554060 @default.
- W1556482919 hasRelatedWork W3135711742 @default.
- W1556482919 hasRelatedWork W4210732568 @default.
- W1556482919 hasRelatedWork W4307000021 @default.
- W1556482919 hasRelatedWork W4312670740 @default.
- W1556482919 hasVolume "9" @default.
- W1556482919 isParatext "false" @default.
- W1556482919 isRetracted "false" @default.
- W1556482919 magId "1556482919" @default.
- W1556482919 workType "article" @default.