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- W1556553433 abstract "Fresh human skin placed in plasma clot culture shows outgrowth of epithelial cells within 3 days followed 3 to 6 days later by fibroblasts. Plasma clot culture of human skin pretreated in medium containing 10% glycerol at 4°C, frozen, and thawed showed outgrowth of fibroblasts only. Using this increased susceptibility of epithelial cells to freeze injury as a criterion, we developed a freezing method in which skin is pretreated with 20 to 30% glycerol at 4°C for 1 to 2 hrs before freezing. Human skin preserved in liquid nitrogen by this method grew out in plasma clot culture very much like fresh skin." @default.
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- W1556553433 date "1969-01-01" @default.
- W1556553433 modified "2023-09-25" @default.
- W1556553433 title "Differential susceptibility of epithelial cells and fibroblasts of human skin to freeze injury" @default.
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- W1556553433 doi "https://doi.org/10.1016/s0011-2240(69)80066-0" @default.
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