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- W1556820106 abstract "Publisher Summary The removal of low-molecular-weight solutes and concentration of protein solutions are the most frequently performed operations in protein purification and analysis. The highly specific characteristics of the individual protein and the requirements of the experiment must be taken into consideration. In most cases, concentrating and desalting of a protein solution can be achieved without substantial losses or effects on the biological activity of the protein. Electrophoretic techniques, ion-exchange chromatography, and protein sequencing are particularly sensitive to the presence of salts and may additionally require concentrated protein samples as is the case in size-exclusion chromatography. Modern technology has provided membranes for ultra-filtration and dialysis allowing the concentration and desalting of proteins with acceptable yields and little denaturing effect. New and improved supports for conventional chromatography have made gel filtration often the method of choice to change the buffer conditions or composition of a protein solution. The first parameter to be considered in a concentration or desalting experiment is the initial volume of the protein sample and the volume that is desired after the procedure. In many cases, concentration and desalting are interlinked elements between different steps of protein purification. Whenever possible, the order of the individual steps of protein separation should be arranged in a way that concentrating techniques follow those methods leading to dilute protein samples." @default.
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- W1556820106 date "1990-01-01" @default.
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- W1556820106 title "[7] Concentration of proteins and removal of solutes" @default.
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- W1556820106 doi "https://doi.org/10.1016/0076-6879(90)82009-q" @default.
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