Matches in SemOpenAlex for { <https://semopenalex.org/work/W1556989342> ?p ?o ?g. }
- W1556989342 endingPage "468" @default.
- W1556989342 startingPage "464" @default.
- W1556989342 abstract "PCR amplification and DNA sequencing of the expression locus from Neisseria gonorrhoeae contained in urine sediments collected from experimentally infected human subjects produced two observations. First, different pilin sequences were obtained when separate aliquots of the same sample were amplified and sequenced. In contrast, the same pilin sequence was obtained when repeated amplifications were performed on individual colonies grown from the clinical samples. Second, mixed sequences (i.e., more than one nucleotide at variable positions in the pilin gene sequence) were observed in both the direct clinical isolates and individual cultures grown from the isolates. These results suggest that when clinical samples are directly examined by PCR amplification and sequencing, multiple amplifications may be required to detect sequence variants in the sample and minority variant sequences will not always be detected." @default.
- W1556989342 created "2016-06-24" @default.
- W1556989342 creator A5004551370 @default.
- W1556989342 creator A5024262342 @default.
- W1556989342 creator A5029543804 @default.
- W1556989342 creator A5055271261 @default.
- W1556989342 creator A5061283368 @default.
- W1556989342 date "1994-02-01" @default.
- W1556989342 modified "2023-09-29" @default.
- W1556989342 title "Nonrepresentative PCR amplification of variable gene sequences in clinical specimens containing dilute, complex mixtures of microorganisms" @default.
- W1556989342 cites W1524005751 @default.
- W1556989342 cites W1933226978 @default.
- W1556989342 cites W1964231265 @default.
- W1556989342 cites W1983307257 @default.
- W1556989342 cites W1984645523 @default.
- W1556989342 cites W2018340379 @default.
- W1556989342 cites W2036801834 @default.
- W1556989342 cites W2083066193 @default.
- W1556989342 cites W2103884702 @default.
- W1556989342 cites W2107195420 @default.
- W1556989342 cites W2128091253 @default.
- W1556989342 cites W2131626832 @default.
- W1556989342 cites W2141752248 @default.
- W1556989342 cites W2147403966 @default.
- W1556989342 cites W2168422276 @default.
- W1556989342 cites W2180855780 @default.
- W1556989342 cites W2222197629 @default.
- W1556989342 cites W2341768065 @default.
- W1556989342 doi "https://doi.org/10.1128/jcm.32.2.464-468.1994" @default.
- W1556989342 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/263055" @default.
- W1556989342 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7908674" @default.
- W1556989342 hasPublicationYear "1994" @default.
- W1556989342 type Work @default.
- W1556989342 sameAs 1556989342 @default.
- W1556989342 citedByCount "31" @default.
- W1556989342 countsByYear W15569893422012 @default.
- W1556989342 countsByYear W15569893422016 @default.
- W1556989342 countsByYear W15569893422017 @default.
- W1556989342 countsByYear W15569893422019 @default.
- W1556989342 countsByYear W15569893422023 @default.
- W1556989342 crossrefType "journal-article" @default.
- W1556989342 hasAuthorship W1556989342A5004551370 @default.
- W1556989342 hasAuthorship W1556989342A5024262342 @default.
- W1556989342 hasAuthorship W1556989342A5029543804 @default.
- W1556989342 hasAuthorship W1556989342A5055271261 @default.
- W1556989342 hasAuthorship W1556989342A5061283368 @default.
- W1556989342 hasBestOaLocation W15569893421 @default.
- W1556989342 hasConcept C104317684 @default.
- W1556989342 hasConcept C153911025 @default.
- W1556989342 hasConcept C174832134 @default.
- W1556989342 hasConcept C2776803919 @default.
- W1556989342 hasConcept C2777954195 @default.
- W1556989342 hasConcept C2778112365 @default.
- W1556989342 hasConcept C2779166590 @default.
- W1556989342 hasConcept C49105822 @default.
- W1556989342 hasConcept C51679486 @default.
- W1556989342 hasConcept C523546767 @default.
- W1556989342 hasConcept C54355233 @default.
- W1556989342 hasConcept C60987743 @default.
- W1556989342 hasConcept C61053724 @default.
- W1556989342 hasConcept C84148353 @default.
- W1556989342 hasConcept C84597430 @default.
- W1556989342 hasConcept C86803240 @default.
- W1556989342 hasConceptScore W1556989342C104317684 @default.
- W1556989342 hasConceptScore W1556989342C153911025 @default.
- W1556989342 hasConceptScore W1556989342C174832134 @default.
- W1556989342 hasConceptScore W1556989342C2776803919 @default.
- W1556989342 hasConceptScore W1556989342C2777954195 @default.
- W1556989342 hasConceptScore W1556989342C2778112365 @default.
- W1556989342 hasConceptScore W1556989342C2779166590 @default.
- W1556989342 hasConceptScore W1556989342C49105822 @default.
- W1556989342 hasConceptScore W1556989342C51679486 @default.
- W1556989342 hasConceptScore W1556989342C523546767 @default.
- W1556989342 hasConceptScore W1556989342C54355233 @default.
- W1556989342 hasConceptScore W1556989342C60987743 @default.
- W1556989342 hasConceptScore W1556989342C61053724 @default.
- W1556989342 hasConceptScore W1556989342C84148353 @default.
- W1556989342 hasConceptScore W1556989342C84597430 @default.
- W1556989342 hasConceptScore W1556989342C86803240 @default.
- W1556989342 hasIssue "2" @default.
- W1556989342 hasLocation W15569893421 @default.
- W1556989342 hasLocation W15569893422 @default.
- W1556989342 hasLocation W15569893423 @default.
- W1556989342 hasLocation W15569893424 @default.
- W1556989342 hasOpenAccess W1556989342 @default.
- W1556989342 hasPrimaryLocation W15569893421 @default.
- W1556989342 hasRelatedWork W1960963758 @default.
- W1556989342 hasRelatedWork W1972681927 @default.
- W1556989342 hasRelatedWork W1995959020 @default.
- W1556989342 hasRelatedWork W2001273620 @default.
- W1556989342 hasRelatedWork W2003638131 @default.
- W1556989342 hasRelatedWork W2020017275 @default.
- W1556989342 hasRelatedWork W2021580933 @default.
- W1556989342 hasRelatedWork W2104498991 @default.
- W1556989342 hasRelatedWork W2113757799 @default.
- W1556989342 hasRelatedWork W2141752248 @default.
- W1556989342 hasVolume "32" @default.
- W1556989342 isParatext "false" @default.