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• W1557024856 endingPage "168" @default.
• W1557024856 startingPage "161" @default.
• W1557024856 abstract "A sulfate-reducing vibrio was isolated from a methanogenic enrichment with choline as the sole added organic substrate. This organism was identified as a member of the genus Desulfovibrio and was designated Desulfovibrio strain G1. In a defined medium devoid of sulfate, a pure culture of Desulfovibrio strain G1 fermented choline to trimethylamine, acetate, and ethanol. In the presence of sulfate, more acetate and less ethanol were formed from choline than in the absence of sulfate. When grown in a medium containing sulfate, a coculture of Desulfovibrio strain G1 and Methanosarcina barkeri strain Fusaro degraded choline almost completely to methane, ammonia, and hydrogen sulfide and presumably to carbon dioxide. Methanogenesis occurred in two distinct phases separated by a lag of about 6 days. During the first phase of methanogenesis choline was completely converted to trimethylamine, acetate, hydrogen sulfide, and traces of ethanol by the desulfovibrio. M. barkeri fermented trimethylamine to methane, ammonia, and presumably carbon dioxide via dimethyl- and methylamine as intermediates. Simultaneously, about 60% of the acetate expected was metabolized. In the second phase of methanogenesis, the residual acetate was almost completely catabolized." @default.
• W1557024856 created "2016-06-24" @default.
• W1557024856 creator A5072930747 @default.
• W1557024856 creator A5083043647 @default.
• W1557024856 date "1983-01-01" @default.
• W1557024856 modified "2023-10-17" @default.
• W1557024856 title "Methanogenesis from Choline by a Coculture of <i>Desulfovibrio</i> sp. and <i>Methanosarcina barkeri</i>" @default.
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• W1557024856 doi "https://doi.org/10.1128/aem.45.1.161-168.1983" @default.
• W1557024856 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/242247" @default.
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