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- W1557191507 abstract "Peptides of the neurointermediate (NI) lobe of the American bullfrog (Rana catesbeiana) were separated by polyacrylamide disc gel electrophoresis (PDGE) and by gel filtration. Those which had melanotropic activity (MSH) were identified using the Anolis skin bioassay. Five forms of MSH were detected on acrylamide gels. One region exhibited characteristics of an acid-activatable aggregate. Two regions had electrophoretic mobilities similar to mammalian α- and β-melanocyte-stimulating hormone. A high molecular weight form, which was also acid activatable, was separated by gel filtration on Sephadex G-50. A secreted form which had electrophoretic properties similar to mammalian β-MSH, was detected by PDGE of medium from a culture of NI lobes for 1 day. Tritiated amino acids were actively incorporated into the secreted form during incubation of NI lobes. These results indicate that the pars intermedia of the bullfrog contains multiple forms of MSH but it secretes predominantly only one form which resembles mammalian β-MSH. The gland contains a high molecular weight form which is converted to smaller forms by acid treatment." @default.
- W1557191507 created "2016-06-24" @default.
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- W1557191507 date "1979-11-01" @default.
- W1557191507 modified "2023-09-24" @default.
- W1557191507 title "Studies on the melanocyte-stimulating hormones of the neurointermediate lobe of the American bullfrog, Rana catesbeiana" @default.
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- W1557191507 doi "https://doi.org/10.1016/0016-6480(79)90126-6" @default.
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