Matches in SemOpenAlex for { <https://semopenalex.org/work/W1557510972> ?p ?o ?g. }
- W1557510972 endingPage "169" @default.
- W1557510972 startingPage "133" @default.
- W1557510972 abstract "Publisher Summary This chapter describes the applications of multidimensional fluorescence imaging (MDFI) instrumentation. The chapter discusses fluorescence lifetime imaging (FLIM), with an emphasis on rapid wide-field time-gated imaging, including application to molecular biology, FLIM of tissue autofluorescence, and highspeed optically sectioned FLIM for live cell imaging. Optical sectioning is important to enhance image contrast and to minimize unwanted mixing of signals from axially separate fluorophores. The chapter discusses the extension to spectral FLIM, including (emission resolved) hyperspectral FLIM, implemented using line-scanning microscopy, and excitation-resolved imaging and FLIM utilizing supercontinuum generation to provide excitation throughout the visible spectrum. The combination of polarization-resolved and time resolved fluorescence imaging is described, mapping both lifetime and rotational correlation time as illustrated by an application to microfluidic devices. This chapter demonstrates that FLIM and MDFI can add significant value to microscopy, endoscopy, and assay technology. By resolving the fluorescence signal with respect to multiple dimensions including excitation and emission wavelength, lifetime, and polarization, it may be possible to enhance the fluorescence read-out for an experiment or assay, for example, in terms of improving the separation of multiple fluorophores or imaging variations in the local molecular environment." @default.
- W1557510972 created "2016-06-24" @default.
- W1557510972 creator A5000206749 @default.
- W1557510972 creator A5005547852 @default.
- W1557510972 creator A5016467870 @default.
- W1557510972 creator A5023098291 @default.
- W1557510972 creator A5034329813 @default.
- W1557510972 creator A5035947212 @default.
- W1557510972 creator A5036859039 @default.
- W1557510972 creator A5042501275 @default.
- W1557510972 creator A5042539600 @default.
- W1557510972 creator A5046668694 @default.
- W1557510972 creator A5048138034 @default.
- W1557510972 creator A5052948415 @default.
- W1557510972 creator A5055381065 @default.
- W1557510972 creator A5056424425 @default.
- W1557510972 creator A5057509807 @default.
- W1557510972 creator A5057936199 @default.
- W1557510972 creator A5062278190 @default.
- W1557510972 creator A5062289075 @default.
- W1557510972 creator A5063428755 @default.
- W1557510972 creator A5067598719 @default.
- W1557510972 creator A5071572939 @default.
- W1557510972 creator A5075978685 @default.
- W1557510972 creator A5081303327 @default.
- W1557510972 creator A5086866155 @default.
- W1557510972 creator A5087398359 @default.
- W1557510972 date "2009-01-01" @default.
- W1557510972 modified "2023-10-18" @default.
- W1557510972 title "Chapter 4 Multidimensional fluorescence imaging" @default.
- W1557510972 cites W1597253918 @default.
- W1557510972 cites W1973252991 @default.
- W1557510972 cites W1975501101 @default.
- W1557510972 cites W1981564484 @default.
- W1557510972 cites W1985749767 @default.
- W1557510972 cites W1985851300 @default.
- W1557510972 cites W1993272328 @default.
- W1557510972 cites W1993759736 @default.
- W1557510972 cites W1994278913 @default.
- W1557510972 cites W1997967093 @default.
- W1557510972 cites W1998506149 @default.
- W1557510972 cites W2000960806 @default.
- W1557510972 cites W2003224117 @default.
- W1557510972 cites W2003861341 @default.
- W1557510972 cites W2004814586 @default.
- W1557510972 cites W2008218710 @default.
- W1557510972 cites W2008645012 @default.
- W1557510972 cites W2009014044 @default.
- W1557510972 cites W2010709858 @default.
- W1557510972 cites W2012530541 @default.
- W1557510972 cites W2017295289 @default.
- W1557510972 cites W2017909074 @default.
- W1557510972 cites W2020556902 @default.
- W1557510972 cites W2022910482 @default.
- W1557510972 cites W2031113704 @default.
- W1557510972 cites W2033076785 @default.
- W1557510972 cites W2038468610 @default.
- W1557510972 cites W2040723705 @default.
- W1557510972 cites W2040863766 @default.
- W1557510972 cites W2042088844 @default.
- W1557510972 cites W2043023303 @default.
- W1557510972 cites W2043529114 @default.
- W1557510972 cites W2053915652 @default.
- W1557510972 cites W2056113960 @default.
- W1557510972 cites W2060015322 @default.
- W1557510972 cites W2061464631 @default.
- W1557510972 cites W2063158873 @default.
- W1557510972 cites W2063965227 @default.
- W1557510972 cites W2066736326 @default.
- W1557510972 cites W2068026071 @default.
- W1557510972 cites W2076815583 @default.
- W1557510972 cites W2079293985 @default.
- W1557510972 cites W2082724001 @default.
- W1557510972 cites W2083668769 @default.
- W1557510972 cites W2084278880 @default.
- W1557510972 cites W2087323736 @default.
- W1557510972 cites W2090663204 @default.
- W1557510972 cites W2092781383 @default.
- W1557510972 cites W2100320779 @default.
- W1557510972 cites W2110384282 @default.
- W1557510972 cites W2115737236 @default.
- W1557510972 cites W2116284076 @default.
- W1557510972 cites W2119299949 @default.
- W1557510972 cites W2123838431 @default.
- W1557510972 cites W2130895233 @default.
- W1557510972 cites W2134991960 @default.
- W1557510972 cites W2146011638 @default.
- W1557510972 cites W2147229775 @default.
- W1557510972 cites W2150286335 @default.
- W1557510972 cites W2152517009 @default.
- W1557510972 cites W2153086900 @default.
- W1557510972 cites W2154287332 @default.
- W1557510972 cites W2154329137 @default.
- W1557510972 cites W2156021298 @default.
- W1557510972 cites W2163127244 @default.
- W1557510972 cites W2166168429 @default.
- W1557510972 cites W2170419840 @default.
- W1557510972 cites W4230350617 @default.