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- W1557714923 abstract "This chapter describes the total internal reflection fluorescence (TIRF) microscopy in cell biology. TIRF microscopy (also called “evanescent wave microscopy”) provides a means to selectively excite fluorophores in an aqueous or cellular environment very near a solid surface, without exciting fluorescence in regions farther from the surface. Fluorescence excitation by this thin zone of electromagnetic energy results in images with very low background fluorescence, virtually no out-of-focus fluorescence, and minimal exposure of cells to light at any other planes in the sample. Another type of intermediate film is now coming into commercial use in bioassay surfaces based on TIRF—the thin film planar waveguide. An ordinary glass (or plastic) surface is etched or embossed with closely spaced grooves that behave like a diffraction grating so that some of the light coming through the glass toward the surface will diffract away from the normal at the surface. Cell-substrate contacts can be located by a non-fluorescence technique completely distinct from TIRF, known as “internal reflection microscopy.”" @default.
- W1557714923 created "2016-06-24" @default.
- W1557714923 creator A5087088078 @default.
- W1557714923 date "2003-01-01" @default.
- W1557714923 modified "2023-10-10" @default.
- W1557714923 title "[1] Total internal reflection fluorescence microscopy in cell biology" @default.
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- W1557714923 doi "https://doi.org/10.1016/s0076-6879(03)61003-7" @default.
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