FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1558020506> ?p ?o ?g. }

• W1558020506 endingPage "4619" @default.
• W1558020506 startingPage "4613" @default.
• W1558020506 abstract "RNA editing in kinetoplastids is the post-transcriptional insertion and deletion of uridylate residues in mitochondrial transcripts, directed by base pairing with guide RNAs. Models for editing propose transesterification or endonuclease plus RNA ligase reactions and may involve a guide RNA-mRNA chimeric intermediate. We have assessed the feasibility of the enzymatic pathway involving chimeras in vitro. Cytochrome b chimeras generated with mitochondrial extract were first found to have junctions primarily at the major endonuclease cleavage sites, supporting the role of endonuclease in chimera formation. Such cytochrome b chimeras are then specifically cleaved by extract endonuclease within the oligo(U) tract at the editing site, and the mRNA cleavage products are then joined by RNA ligase to generate partially edited mRNAs with uridylate residues transferred to an editing site. These in vitro generated partially edited mRNAs mimic partially edited mRNAs generated in vivo. Specific endonuclease cleavage in the editing region of the partially edited RNA demonstrates the potential for further in vitro editing. Finally, sensitivity to various ATP analogs suggests that all editing-like activities reported thus far utilize a mechanism involving RNA ligase. RNA editing in kinetoplastids is the post-transcriptional insertion and deletion of uridylate residues in mitochondrial transcripts, directed by base pairing with guide RNAs. Models for editing propose transesterification or endonuclease plus RNA ligase reactions and may involve a guide RNA-mRNA chimeric intermediate. We have assessed the feasibility of the enzymatic pathway involving chimeras in vitro. Cytochrome b chimeras generated with mitochondrial extract were first found to have junctions primarily at the major endonuclease cleavage sites, supporting the role of endonuclease in chimera formation. Such cytochrome b chimeras are then specifically cleaved by extract endonuclease within the oligo(U) tract at the editing site, and the mRNA cleavage products are then joined by RNA ligase to generate partially edited mRNAs with uridylate residues transferred to an editing site. These in vitro generated partially edited mRNAs mimic partially edited mRNAs generated in vivo. Specific endonuclease cleavage in the editing region of the partially edited RNA demonstrates the potential for further in vitro editing. Finally, sensitivity to various ATP analogs suggests that all editing-like activities reported thus far utilize a mechanism involving RNA ligase." @default.
• W1558020506 created "2016-06-24" @default.
• W1558020506 creator A5011823990 @default.
• W1558020506 creator A5037717192 @default.
• W1558020506 creator A5062409481 @default.
• W1558020506 date "1996-03-01" @default.
• W1558020506 modified "2023-10-02" @default.
• W1558020506 title "Trypanosoma brucei RNA Editing" @default.
• W1558020506 cites W1607243293 @default.
• W1558020506 cites W1742656526 @default.
• W1558020506 cites W1790527661 @default.
• W1558020506 cites W1865319854 @default.
• W1558020506 cites W1892019602 @default.
• W1558020506 cites W1934766143 @default.
• W1558020506 cites W1966857238 @default.
• W1558020506 cites W1969769090 @default.
• W1558020506 cites W1971014384 @default.
• W1558020506 cites W1973423766 @default.
• W1558020506 cites W1975920595 @default.
• W1558020506 cites W1981419683 @default.
• W1558020506 cites W1982966054 @default.
• W1558020506 cites W1987956539 @default.
• W1558020506 cites W1993742413 @default.
• W1558020506 cites W2000775841 @default.
• W1558020506 cites W2025763720 @default.
• W1558020506 cites W2041859826 @default.
• W1558020506 cites W2060535275 @default.
• W1558020506 cites W2061420032 @default.
• W1558020506 cites W2068683905 @default.
• W1558020506 cites W2080653091 @default.
• W1558020506 cites W2084211101 @default.
• W1558020506 cites W2128972342 @default.
• W1558020506 cites W2142471561 @default.
• W1558020506 cites W2143653514 @default.
• W1558020506 cites W2145594096 @default.
• W1558020506 cites W2151424128 @default.
• W1558020506 cites W2166178374 @default.
• W1558020506 cites W2170107692 @default.
• W1558020506 cites W2170224780 @default.
• W1558020506 cites W2264429446 @default.
• W1558020506 cites W4234510935 @default.
• W1558020506 cites W968389318 @default.
• W1558020506 doi "https://doi.org/10.1074/jbc.271.9.4613" @default.
• W1558020506 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8617722" @default.
• W1558020506 hasPublicationYear "1996" @default.
• W1558020506 type Work @default.
• W1558020506 sameAs 1558020506 @default.
• W1558020506 citedByCount "12" @default.
• W1558020506 crossrefType "journal-article" @default.
• W1558020506 hasAuthorship W1558020506A5011823990 @default.
• W1558020506 hasAuthorship W1558020506A5037717192 @default.
• W1558020506 hasAuthorship W1558020506A5062409481 @default.
• W1558020506 hasBestOaLocation W15580205061 @default.
• W1558020506 hasConcept C104317684 @default.
• W1558020506 hasConcept C144501496 @default.
• W1558020506 hasConcept C153911025 @default.
• W1558020506 hasConcept C175114707 @default.
• W1558020506 hasConcept C21786251 @default.
• W1558020506 hasConcept C2777028655 @default.
• W1558020506 hasConcept C2909068217 @default.
• W1558020506 hasConcept C552990157 @default.
• W1558020506 hasConcept C55493867 @default.
• W1558020506 hasConcept C67705224 @default.
• W1558020506 hasConcept C86803240 @default.
• W1558020506 hasConcept C95444343 @default.
• W1558020506 hasConcept C97702854 @default.
• W1558020506 hasConcept C98108389 @default.
• W1558020506 hasConceptScore W1558020506C104317684 @default.
• W1558020506 hasConceptScore W1558020506C144501496 @default.
• W1558020506 hasConceptScore W1558020506C153911025 @default.
• W1558020506 hasConceptScore W1558020506C175114707 @default.
• W1558020506 hasConceptScore W1558020506C21786251 @default.
• W1558020506 hasConceptScore W1558020506C2777028655 @default.
• W1558020506 hasConceptScore W1558020506C2909068217 @default.
• W1558020506 hasConceptScore W1558020506C552990157 @default.
• W1558020506 hasConceptScore W1558020506C55493867 @default.
• W1558020506 hasConceptScore W1558020506C67705224 @default.
• W1558020506 hasConceptScore W1558020506C86803240 @default.
• W1558020506 hasConceptScore W1558020506C95444343 @default.
• W1558020506 hasConceptScore W1558020506C97702854 @default.
• W1558020506 hasConceptScore W1558020506C98108389 @default.
• W1558020506 hasIssue "9" @default.
• W1558020506 hasLocation W15580205061 @default.
• W1558020506 hasOpenAccess W1558020506 @default.
• W1558020506 hasPrimaryLocation W15580205061 @default.
• W1558020506 hasRelatedWork W1522635018 @default.
• W1558020506 hasRelatedWork W1790527661 @default.
• W1558020506 hasRelatedWork W1965836951 @default.
• W1558020506 hasRelatedWork W2008591193 @default.
• W1558020506 hasRelatedWork W2127169344 @default.
• W1558020506 hasRelatedWork W2264429446 @default.
• W1558020506 hasRelatedWork W2756473472 @default.
• W1558020506 hasRelatedWork W4243324746 @default.
• W1558020506 hasRelatedWork W2184985809 @default.
• W1558020506 hasRelatedWork W2588789691 @default.
• W1558020506 hasVolume "271" @default.
• W1558020506 isParatext "false" @default.
• W1558020506 isRetracted "false" @default.

• next