Matches in SemOpenAlex for { <https://semopenalex.org/work/W1558060030> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1558060030 endingPage "26721" @default.
- W1558060030 startingPage "26717" @default.
- W1558060030 abstract "The cortical collecting duct of the kidney contains two types of intercalated cells that transport HCO3 in opposite directions. HCO3 reabsorption takes place in the alpha-type intercalated cells, which express a Cl/HCO3 exchanger on the basolateral membrane. This exchanger is the product of the anion exchanger 1 (AE1) or band 3 gene. HCO3 secretion occurs in the beta-intercalated cells, which have a Cl/HCO3 exchanger on the apical membrane. Based on studies in an immortalized cell line, recently it was proposed that the apical anion exchanger of beta-intercalated cells is also AE1 (van Adelsberg, J. S., Edwards, J. C., and Al-Awqati, Q. (1993) J. Biol. Chem. 268, 11283-11289). In the present study we reinvestigated this issue by determining the distribution of AE1 mRNA and protein in the two intercalated cell types using cells freshly isolated from the native epithelium. Using quantitative reverse transcriptase polymerase chain reaction, we found that alpha-intercalated cells, isolated from rabbit kidney by fluorescence-activated cell sorting, have high levels of AE1 mRNA, whereas beta-intercalated cells express very low levels. The ratio of AE1 mRNA levels in alpha- versus beta-intercalated cells averaged 10.1 +/- 2.6. In addition, metabolic acidosis increased the levels of AE1 mRNA by 3-5-fold in cortical collecting duct cells. This difference was confirmed by Northern blotting. Western blotting using an antibody against rabbit AE1 revealed a major immunoreactive product with a molecular weight of approximately 110 kDa in cortical collecting duct cells. Deglycosylation reduced the size of the immunoreactive product to approximately 90 kDa, which is compatible with the presence of a truncated form of AE1. Metabolic acidosis increased the intensity of the AE1 immunoreactive band. The level of AE1-immunoreactive protein was significantly higher in alpha-intercalated cells than in beta-intercalated cells. In aggregate, these data provide evidence for the differential expression of AE1 in HCO3-reabsorbing versus HCO3-secreting renal intercalated cells both at the mRNA and at the protein level. These results give no support to the concept that AE1 functions both as a basolateral and an apical anion exchanger in cortical collecting duct cells." @default.
- W1558060030 created "2016-06-24" @default.
- W1558060030 creator A5001390749 @default.
- W1558060030 creator A5005034773 @default.
- W1558060030 creator A5036064338 @default.
- W1558060030 creator A5077499695 @default.
- W1558060030 creator A5078251690 @default.
- W1558060030 date "1994-10-01" @default.
- W1558060030 modified "2023-10-17" @default.
- W1558060030 title "Differential expression of AE1 in renal HCO3-secreting and -reabsorbing intercalated cells." @default.
- W1558060030 cites W1546193803 @default.
- W1558060030 cites W1551319859 @default.
- W1558060030 cites W1559490361 @default.
- W1558060030 cites W1560118688 @default.
- W1558060030 cites W1974802936 @default.
- W1558060030 cites W1976503461 @default.
- W1558060030 cites W1983837942 @default.
- W1558060030 cites W1984635589 @default.
- W1558060030 cites W1984755904 @default.
- W1558060030 cites W1986001368 @default.
- W1558060030 cites W1996071272 @default.
- W1558060030 cites W2027092642 @default.
- W1558060030 cites W2062045183 @default.
- W1558060030 cites W2071315089 @default.
- W1558060030 cites W2076028855 @default.
- W1558060030 cites W2174130172 @default.
- W1558060030 cites W2174824915 @default.
- W1558060030 cites W2311290643 @default.
- W1558060030 cites W2412669305 @default.
- W1558060030 doi "https://doi.org/10.1016/s0021-9258(18)47078-0" @default.
- W1558060030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7929405" @default.
- W1558060030 hasPublicationYear "1994" @default.
- W1558060030 type Work @default.
- W1558060030 sameAs 1558060030 @default.
- W1558060030 citedByCount "59" @default.
- W1558060030 countsByYear W15580600302012 @default.
- W1558060030 countsByYear W15580600302013 @default.
- W1558060030 countsByYear W15580600302016 @default.
- W1558060030 countsByYear W15580600302017 @default.
- W1558060030 countsByYear W15580600302018 @default.
- W1558060030 countsByYear W15580600302019 @default.
- W1558060030 countsByYear W15580600302020 @default.
- W1558060030 countsByYear W15580600302023 @default.
- W1558060030 crossrefType "journal-article" @default.
- W1558060030 hasAuthorship W1558060030A5001390749 @default.
- W1558060030 hasAuthorship W1558060030A5005034773 @default.
- W1558060030 hasAuthorship W1558060030A5036064338 @default.
- W1558060030 hasAuthorship W1558060030A5077499695 @default.
- W1558060030 hasAuthorship W1558060030A5078251690 @default.
- W1558060030 hasBestOaLocation W15580600301 @default.
- W1558060030 hasConcept C121332964 @default.
- W1558060030 hasConcept C185592680 @default.
- W1558060030 hasConcept C86803240 @default.
- W1558060030 hasConcept C93226319 @default.
- W1558060030 hasConcept C95444343 @default.
- W1558060030 hasConcept C97355855 @default.
- W1558060030 hasConceptScore W1558060030C121332964 @default.
- W1558060030 hasConceptScore W1558060030C185592680 @default.
- W1558060030 hasConceptScore W1558060030C86803240 @default.
- W1558060030 hasConceptScore W1558060030C93226319 @default.
- W1558060030 hasConceptScore W1558060030C95444343 @default.
- W1558060030 hasConceptScore W1558060030C97355855 @default.
- W1558060030 hasIssue "43" @default.
- W1558060030 hasLocation W15580600301 @default.
- W1558060030 hasOpenAccess W1558060030 @default.
- W1558060030 hasPrimaryLocation W15580600301 @default.
- W1558060030 hasRelatedWork W1531601525 @default.
- W1558060030 hasRelatedWork W2319480705 @default.
- W1558060030 hasRelatedWork W2384464875 @default.
- W1558060030 hasRelatedWork W2606230654 @default.
- W1558060030 hasRelatedWork W2607424097 @default.
- W1558060030 hasRelatedWork W2748952813 @default.
- W1558060030 hasRelatedWork W2899084033 @default.
- W1558060030 hasRelatedWork W2948807893 @default.
- W1558060030 hasRelatedWork W4387497383 @default.
- W1558060030 hasRelatedWork W2778153218 @default.
- W1558060030 hasVolume "269" @default.
- W1558060030 isParatext "false" @default.
- W1558060030 isRetracted "false" @default.
- W1558060030 magId "1558060030" @default.
- W1558060030 workType "article" @default.