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- W1558391604 abstract "This unit describes a protocol for the isolation of Drosophila intestinal cell populations for the purpose of cell type-specific transcriptome profiling. A method to select a cell type of interest labeled with green or yellow fluorescent protein (GFP, YFP) by making use of the GAL4-UAS bipartite system and fluorescent-activated cell sorting (FACS) is presented. Total RNA is isolated from the sorted cells and linear RNA amplification is used to obtain sufficient amounts of high-quality RNA for analysis by microarray, RT-PCR, or RNA sequencing. This method will be useful for quantitative transcriptome comparison across intestinal cell types under normal and various experimental conditions." @default.
- W1558391604 created "2016-06-24" @default.
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- W1558391604 date "2013-11-01" @default.
- W1558391604 modified "2023-09-24" @default.
- W1558391604 title "RNA Expression Profiling from FACS‐Isolated Cells of the <i>Drosophila</i> Intestine" @default.
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- W1558391604 doi "https://doi.org/10.1002/9780470151808.sc02f02s27" @default.
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