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- W1560367431 abstract "The addition of NaSCN to Rhodospirillum rubrum chromatophores resulted in a stimulation of the proton uptake and of the light-induced quenching of atebrin fluorescence and in an inhibition of the light-induced enhancement of 8-anilinonaphthalene-1-sulfonic acid fluorescence. These effects were saturated by 10 mm NaSCN, a concentration which did not affect photophosphorylation nor light-induced reduction of NAD+. In the presence of NH4SCN all the tested light-induced reactions in chromatophores were uncoupled. Effects similar to those observed with thiocyanate were also obtained with perchlorate but not with sulfate. Na2SO4 was found to decrease the phosphorylation without affecting any of the other light-induced reactions tested and, like oligomycin, it relieved the inhibition of NAD+ reduction by ADP and inorganic phosphate. (NH4)2SO4, like NH4Cl, inhibited the quenching of atebrin fluorescence, stimulated the enhancement of ANS fluorescence, and only slightly decreased the reduction of NAD+. ATP formation was, however, inhibited by (NH4)2SO4 as well as by Na2SO4. These results are interpreted as indicating that sulfate does not react as a permeant anion in the chromatophores and its inhibition of ATP formation is not due to uncoupling but probably to a block in energy transfer. Thiocyanate and perchlorate on the other hand behave as permeant anions in the chromatophores and indeed in the presence of their sodium salts the pH gradient rather than the membrane potential was found to be the dominant component of the electrochemical proton gradient." @default.
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- W1560367431 date "1973-12-01" @default.
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- W1560367431 title "Interchangeability of the membrane potential with the pH gradient in Rhodospirillum rubrum chromatophores" @default.
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- W1560367431 doi "https://doi.org/10.1016/0003-9861(73)90494-3" @default.
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