Matches in SemOpenAlex for { <https://semopenalex.org/work/W156048355> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W156048355 abstract "Publisher Summary This chapter discusses the affinity chromatography of minimal amount of acetylcholinesterase from nervous tissues and erythrocyte membranes. It discusses a study in which a suitable method for the selective isolation of AChE from other proteins, by affinity chromatography, was worked out by integrating and modifying three available methods. Affigel 202 (Bio-Rad) was covalently linked to a specific and reversible AChE inhibitor. This inhibitor was synthesized, and its properties as the differential inhibitor of AChE and butyrilcholinesterase (3.1.1.8.) (BuChE) were examined. The values of PI50 were found to be 3.8, 4.0, and 2.0 for rat brain AChE, electric eel AChE, and horse serum BuChE, respectively. According to the batchwise procedure, finally adopted, the sample containing at least 60 mU was loaded onto a similar volume of the affinity chromatography gel and the unbound proteins were removed by centrifugation. After three washings with a threefold volume of saline phosphate buffer, AChE was eluted in two steps from the gel by choline chloride solution, which proved effective because of the poor affinity of the linked inhibitor for AChE. The AChE activity, recovered after centrifugation and dialysis of the supernatant, was about 90% of the activity in the sample." @default.
- W156048355 created "2016-06-24" @default.
- W156048355 creator A5025405400 @default.
- W156048355 creator A5041963716 @default.
- W156048355 date "1980-01-01" @default.
- W156048355 modified "2023-09-24" @default.
- W156048355 title "AFFINITY CHROMATOGRAPHY OF MINIMAL AMOUNT OF ACETYLCHOLINESTERASE FROM NERVOUS TISSUES AND ERYTHROCYTE MEMBRANES" @default.
- W156048355 cites W1984960342 @default.
- W156048355 cites W2093766311 @default.
- W156048355 doi "https://doi.org/10.1016/b978-0-08-025921-5.50206-x" @default.
- W156048355 hasPublicationYear "1980" @default.
- W156048355 type Work @default.
- W156048355 sameAs 156048355 @default.
- W156048355 citedByCount "0" @default.
- W156048355 crossrefType "book-chapter" @default.
- W156048355 hasAuthorship W156048355A5025405400 @default.
- W156048355 hasAuthorship W156048355A5041963716 @default.
- W156048355 hasConcept C132946820 @default.
- W156048355 hasConcept C179247698 @default.
- W156048355 hasConcept C179998833 @default.
- W156048355 hasConcept C181199279 @default.
- W156048355 hasConcept C185592680 @default.
- W156048355 hasConcept C2778816929 @default.
- W156048355 hasConcept C2779059548 @default.
- W156048355 hasConcept C41625074 @default.
- W156048355 hasConcept C43617362 @default.
- W156048355 hasConcept C55493867 @default.
- W156048355 hasConcept C73826308 @default.
- W156048355 hasConcept C7623868 @default.
- W156048355 hasConceptScore W156048355C132946820 @default.
- W156048355 hasConceptScore W156048355C179247698 @default.
- W156048355 hasConceptScore W156048355C179998833 @default.
- W156048355 hasConceptScore W156048355C181199279 @default.
- W156048355 hasConceptScore W156048355C185592680 @default.
- W156048355 hasConceptScore W156048355C2778816929 @default.
- W156048355 hasConceptScore W156048355C2779059548 @default.
- W156048355 hasConceptScore W156048355C41625074 @default.
- W156048355 hasConceptScore W156048355C43617362 @default.
- W156048355 hasConceptScore W156048355C55493867 @default.
- W156048355 hasConceptScore W156048355C73826308 @default.
- W156048355 hasConceptScore W156048355C7623868 @default.
- W156048355 hasLocation W1560483551 @default.
- W156048355 hasOpenAccess W156048355 @default.
- W156048355 hasPrimaryLocation W1560483551 @default.
- W156048355 hasRelatedWork W1501153189 @default.
- W156048355 hasRelatedWork W1513395677 @default.
- W156048355 hasRelatedWork W1852321512 @default.
- W156048355 hasRelatedWork W1998215516 @default.
- W156048355 hasRelatedWork W2003617630 @default.
- W156048355 hasRelatedWork W2016077305 @default.
- W156048355 hasRelatedWork W2022562575 @default.
- W156048355 hasRelatedWork W2042135300 @default.
- W156048355 hasRelatedWork W2049253950 @default.
- W156048355 hasRelatedWork W2070855519 @default.
- W156048355 hasRelatedWork W2086019708 @default.
- W156048355 hasRelatedWork W2086427594 @default.
- W156048355 hasRelatedWork W2091397050 @default.
- W156048355 hasRelatedWork W2093766311 @default.
- W156048355 hasRelatedWork W2095175535 @default.
- W156048355 hasRelatedWork W2172084664 @default.
- W156048355 hasRelatedWork W2231440914 @default.
- W156048355 hasRelatedWork W2384896594 @default.
- W156048355 hasRelatedWork W2385714218 @default.
- W156048355 hasRelatedWork W2464089389 @default.
- W156048355 isParatext "false" @default.
- W156048355 isRetracted "false" @default.
- W156048355 magId "156048355" @default.
- W156048355 workType "book-chapter" @default.