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• W1560496206 abstract "Fusion of inducible degradation signals, so-called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin-inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR-based genomic tagging strategies, allow for C- or N-terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. © 2013 The Authors. Yeast published by John Wiley & Sons, Ltd." @default.
• W1560496206 created "2016-06-24" @default.
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• W1560496206 date "2013-07-23" @default.
• W1560496206 modified "2023-10-10" @default.
• W1560496206 title "An expanded tool kit for the auxin‐inducible degron system in budding yeast" @default.
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• W1560496206 doi "https://doi.org/10.1002/yea.2967" @default.
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