FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1560734798> ?p ?o ?g. }

• W1560734798 endingPage "22817" @default.
• W1560734798 startingPage "22807" @default.
• W1560734798 abstract "Saccharomyces SUC2 invertase, secreted by the methylotrophic yeast Pichia pastoris and purified to homogeneity from the growth medium by DE-52 chromatography, appeared on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a diffuse ladder of species at 85-90 kDa, while the secreted Saccharomyces form migrated as a broad band from 100 to 150 kDa. Endo-beta-N-acetylglucosaminidase H released the Pichia invertase carbohydrate generating a 60-kDa protein with residual Asn-linked GlcNAcs and oligosaccharides separated on Bio-Gel P-4 into Man8-11GlcNAc. Nearly 75% of the oligosaccharides were equally distributed between Man8,9GlcNAc, while 17% were Man10GlcNAc and 8% were Man11GlcNAc. Oligosaccharide pools were analyzed for homogeneity by high-pH anion-exchange chromatography, and structures were assigned using 500 MHz one- and two-dimensional 1H NMR spectroscopy. Pichia Man8GlcNAc was the same isomer as found in Saccharomyces, which arises by removing the alpha 1,2-linked terminal mannose from the middle arm of the lipid-oligosaccharide Man9GlcNAc (Byrd, J. C., Tarentino, A. L., Maley, F., Atkinson, P. H., and Trimble, R. B. (1982) J. Biol. Chem. 257, 14657-14666). The Man9GlcNAc pool was 5% lipid-oligosaccharide precursor and 95% Man8GlcNAc isomer with a terminal alpha 1,6-linked mannose on the lower-arm alpha 1,3-core-linked residue (Hernandez, L. M., Ballou, L., Alvarado, E., Gillece-Castro, B. L., Burlingame, A. L., and Ballou, C. E. (1989) J. Biol. Chem. 264, 11849-11856). An alpha 1,2-linked mannose on the new alpha 1,6-linked branch in Man9GlcNAc provided 80% of the Man10GlcNAc, which is the structure on Saccharomyces invertase (Trimble, R. B., and Atkinson, P. H. (1986) J. Biol. Chem. 261, 9815-9824). A minor Man10GlcNAc (12%) and the principal Man11GlcNAc (82%) were the major Man9,10GlcNAc with novel alpha 1,2-linked mannoses on the preexisting alpha 1,2-linked termini. Although Pichia glycans did not have terminal alpha 1,3-linked mannoses as found on Saccharomyces core oligosaccharides, over 60% of the structures were isometric configurations unique to lower eukaryotes." @default.
• W1560734798 created "2016-06-24" @default.
• W1560734798 creator A5008755693 @default.
• W1560734798 creator A5023070360 @default.
• W1560734798 creator A5058305680 @default.
• W1560734798 creator A5061057831 @default.
• W1560734798 creator A5068505847 @default.
• W1560734798 date "1991-12-01" @default.
• W1560734798 modified "2023-10-14" @default.
• W1560734798 title "Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris." @default.
• W1560734798 cites W103713913 @default.
• W1560734798 cites W1489107709 @default.
• W1560734798 cites W1492568021 @default.
• W1560734798 cites W1523022702 @default.
• W1560734798 cites W1544838948 @default.
• W1560734798 cites W154635942 @default.
• W1560734798 cites W1569582665 @default.
• W1560734798 cites W1584526531 @default.
• W1560734798 cites W1590585236 @default.
• W1560734798 cites W1594958145 @default.
• W1560734798 cites W1609122418 @default.
• W1560734798 cites W1611254900 @default.
• W1560734798 cites W1968834637 @default.
• W1560734798 cites W1975699819 @default.
• W1560734798 cites W2008765994 @default.
• W1560734798 cites W2018825404 @default.
• W1560734798 cites W2023246467 @default.
• W1560734798 cites W2025604503 @default.
• W1560734798 cites W2037366996 @default.
• W1560734798 cites W2053590990 @default.
• W1560734798 cites W2054989937 @default.
• W1560734798 cites W2056509744 @default.
• W1560734798 cites W2068109582 @default.
• W1560734798 cites W2087681334 @default.
• W1560734798 cites W2100837269 @default.
• W1560734798 cites W2113060696 @default.
• W1560734798 doi "https://doi.org/10.1016/s0021-9258(18)54426-4" @default.
• W1560734798 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1744075" @default.
• W1560734798 hasPublicationYear "1991" @default.
• W1560734798 type Work @default.
• W1560734798 sameAs 1560734798 @default.
• W1560734798 citedByCount "137" @default.
• W1560734798 countsByYear W15607347982012 @default.
• W1560734798 countsByYear W15607347982013 @default.
• W1560734798 countsByYear W15607347982014 @default.
• W1560734798 countsByYear W15607347982015 @default.
• W1560734798 countsByYear W15607347982017 @default.
• W1560734798 countsByYear W15607347982019 @default.
• W1560734798 countsByYear W15607347982021 @default.
• W1560734798 countsByYear W15607347982022 @default.
• W1560734798 crossrefType "journal-article" @default.
• W1560734798 hasAuthorship W1560734798A5008755693 @default.
• W1560734798 hasAuthorship W1560734798A5023070360 @default.
• W1560734798 hasAuthorship W1560734798A5058305680 @default.
• W1560734798 hasAuthorship W1560734798A5061057831 @default.
• W1560734798 hasAuthorship W1560734798A5068505847 @default.
• W1560734798 hasBestOaLocation W15607347981 @default.
• W1560734798 hasConcept C104317684 @default.
• W1560734798 hasConcept C181199279 @default.
• W1560734798 hasConcept C185592680 @default.
• W1560734798 hasConcept C2777576037 @default.
• W1560734798 hasConcept C2778252681 @default.
• W1560734798 hasConcept C2778447961 @default.
• W1560734798 hasConcept C2779222958 @default.
• W1560734798 hasConcept C2779703480 @default.
• W1560734798 hasConcept C2781372317 @default.
• W1560734798 hasConcept C40767141 @default.
• W1560734798 hasConcept C55493867 @default.
• W1560734798 hasConceptScore W1560734798C104317684 @default.
• W1560734798 hasConceptScore W1560734798C181199279 @default.
• W1560734798 hasConceptScore W1560734798C185592680 @default.
• W1560734798 hasConceptScore W1560734798C2777576037 @default.
• W1560734798 hasConceptScore W1560734798C2778252681 @default.
• W1560734798 hasConceptScore W1560734798C2778447961 @default.
• W1560734798 hasConceptScore W1560734798C2779222958 @default.
• W1560734798 hasConceptScore W1560734798C2779703480 @default.
• W1560734798 hasConceptScore W1560734798C2781372317 @default.
• W1560734798 hasConceptScore W1560734798C40767141 @default.
• W1560734798 hasConceptScore W1560734798C55493867 @default.
• W1560734798 hasIssue "34" @default.
• W1560734798 hasLocation W15607347981 @default.
• W1560734798 hasOpenAccess W1560734798 @default.
• W1560734798 hasPrimaryLocation W15607347981 @default.
• W1560734798 hasRelatedWork W1480951157 @default.
• W1560734798 hasRelatedWork W1975687798 @default.
• W1560734798 hasRelatedWork W2073938168 @default.
• W1560734798 hasRelatedWork W2120842154 @default.
• W1560734798 hasRelatedWork W2256808269 @default.
• W1560734798 hasRelatedWork W2368039950 @default.
• W1560734798 hasRelatedWork W2393867567 @default.
• W1560734798 hasRelatedWork W2394330041 @default.
• W1560734798 hasRelatedWork W2414398983 @default.
• W1560734798 hasRelatedWork W2623087137 @default.
• W1560734798 hasVolume "266" @default.
• W1560734798 isParatext "false" @default.
• W1560734798 isRetracted "false" @default.
• W1560734798 magId "1560734798" @default.
• W1560734798 workType "article" @default.