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- W1560914011 abstract "AIM: To establish a convenient and efficient transformation system of the marine-sourced filamentous fungus Phoma herbarum YS4108. METHODS: The endogenous actin promoter was cloned with degenerate primers and genome walking strategy, and utilized in the electroporation of P. herbarum YS4108. Using hygromycin B phosphotransferase gene as a selection marker and single cells prepared from mycelia as recipients, the effect of electroporation parameters on transformation efficiency was examined. RESULTS: The actin promoter was successfully cloned. The optimum parameters for electroporation were found to be 0.75 kV (0.2 cm cuvette) in voltage and 400Ω in resistance with 9μg of plasmid, under which more than 100 transformants per cuvette (10^6 recipients) could be obtained. CONCLUSION: By using the endogenous actin promoter, the erectric transformation system of P. herbarum YS4108 was established, which served as a practical approach for transgenic study on this fungus." @default.
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- W1560914011 date "2008-07-01" @default.
- W1560914011 modified "2023-09-25" @default.
- W1560914011 title "Cloning of actin Promoter and Transformation of the Marine-Sourced Filamentous Fungus Phoma herbarum YS4108" @default.
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- W1560914011 doi "https://doi.org/10.1016/s1875-5364(09)60026-1" @default.
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