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- W1560920782 abstract "Abstract: The effect of the insecticide methoxychlor on the physiology of oral cells is unknown. This study aimed to explore the effect of methoxychlor on cytosolic Ca 2+ concentrations ([Ca 2+ ] i ) in human oral cancer cells (OC2) by using the Ca 2+ ‐sensitive fluorescent dye fura‐2. Methoxychlor at 5–20 μM increased [Ca 2+ ] i in a concentration‐dependent manner. The signal was reduced by 70% by removing extracellular Ca 2+ . Methoxychlor‐induced Ca 2+ entry was not affected by nifedipine, econazole, SK&F96365 and protein kinase C modulators but was inhibited by the phospholipase A2 inhibitor aristolochic acid. In Ca 2+ ‐free medium, treatment with the endoplasmic reticulum Ca 2+ pump inhibitor thapsigargin or 2,5‐di‐tert‐butylhydroquinone (BHQ) inhibited or abolished methoxychlor‐induced [Ca 2+ ] i rise. Incubation with methoxychlor also inhibited thapsigargin‐ or BHQ‐induced [Ca 2+ ] i rise. Inhibition of phospholipase C with U73122 did not alter methoxychlor‐induced [Ca 2+ ] i rise. At 5–20 μM, methoxychlor killed cells in a concentration‐dependent manner. The cytotoxic effect of methoxychlor was not reversed by chelating cytosolic Ca 2+ with 1,2‐bis(2‐aminophenoxy)ethane‐N,N,N’,N’‐tetraacetic acid/AM (BAPTA/AM). Annexin V‐FITC data suggest that methoxychlor (10 and 20 μM) evoked apoptosis in a concentration‐dependent manner. Together, in human OC2, methoxychlor induced a [Ca 2+ ] i rise probably by inducing phospholipase C‐independent Ca 2+ release from the endoplasmic reticulum and Ca 2+ entry via phospholipase A 2 ‐sensitive channels. Methoxychlor induced cell death that may involve apoptosis." @default.
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- W1560920782 date "2011-01-10" @default.
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- W1560920782 title "Effect of Methoxychlor on Ca2+ Handling and Viability in OC2 Human Oral Cancer Cells" @default.
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- W1560920782 doi "https://doi.org/10.1111/j.1742-7843.2010.00662.x" @default.
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