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- W1560935512 abstract "Drosophila is a model genus that can be used to improve our understanding of mammalian spermatogenesis as the cellular and molecular processes in mammalian and fruit fly spermatogenesis are very similar. In Drosophila pseudoabscura spermatogenesis, 128 round spermatids arise via five mitotic and two meiotic divisions within an encapsulating cyst and then transform into mature, elongate sperm during a post-meiotic stage termed spermiogenesis. Prior to spermiogenesis, the 128 spermatid nuclei relocate to one side of the cyst, however the mechanism by which this relocation occurs is unknown. Cytoskeletal and motor proteins have been reported to be responsible for different nuclear migration events across biological systems. Because actin structures are involved in later stages of sperm maturation, we hypothesized that an actin-based structure may be responsible for the nuclear migration event. Also, actin has been investigated in nuclear migration events in different models including Drosophila, and results prove it is an important player. Our results indicate that the nuclei in the 128-cell stage relocate to one side of the cyst during spermatogenesis in D. pseudoobscura. Also, we report the presence of a distinct, spherical actin-based structure associated with the sperm nuclei in pre-migratory 128-cell cysts. The nuclei appear to congregate around this structure prior to migration. Furthermore, actin is detected as a punctate cytoplasmic signal prior to this stage. Cell culture techniques were also used to investigate D. pseudoobscura sperm cell viability after 24 hours. These cysts were stained with Hoechst 33342 at the beginning of the culture, and tested for viability by calcein-AM staining. Interestingly, we detected multiple cytoplasmic lelongation bulges' in early post migration cysts using this protocol. Multiple bulges were detected on each developing sperm precursor before the nuclei had undergone nuclear migration. Also, cytoplasmic Ivoids' were detected within the head cyst cells associated with this stage; the function of these structures is unknown." @default.
- W1560935512 created "2016-06-24" @default.
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- W1560935512 date "2012-01-01" @default.
- W1560935512 modified "2023-09-27" @default.
- W1560935512 title "Nuclear Migration and Spermatid Elongation During Spermiogenesis in Drosophila Pseudoobscura" @default.
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