FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1561261068> ?p ?o ?g. }

• W1561261068 abstract "The potential effects of introducing bone regeneration strategies into environments of disease and damage are often overlooked, despite the fact that many of the signalling pathways in inflammation have effects on bone development and healing. Embryonic stem cells (ESCs) are increasingly being used to develop models of disease and have potential in osteogenic-cell based therapies. Osteogenic differentiation strategies for ESCs are well established, but the response of these cells to tissue damage and inflammation has not yet been investigated, particularly in comparison to primary osteoblasts. Here, proinflammatory cytokines were used as part of an in vitro model to mimic elements of skeletal disease, such as rheumatoid arthritis and non-union fractures. The response of osteogenically differentiated mouse embryonic stem cells (osteo-mESCs) to the proinflammatory cytokines interleukin 1-β (IL-1β), tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), was compared to that of primary mouse calvarial osteoblasts, already well-described in literature and used as a “benchmark” in this study. Although histology, immunocytochemistry and PCR showed similarities in osteogenic differentiation of the osteo-mESCs and the primary calvarial cells, over 21 days in culture, there were marked differences in the response to the proinflammatory cytokines. Viability of the osteo-mESCs was maintained in response to cytokines, whereas viability of primary cells was significantly reduced. There were marked increases in nitric oxide (NO) and prostaglandin E2 (PGE2) production in primary calvarial cells over the entire 21-day culture period, but this was not seen with osteo-mESCs until day 21. The study then went on to look at the effects of proinflammatory signalling on the in vitro bone formation of the two cell types. Significant differences in the effects of proinflammatory cytokines on bone nodule formation and matrix production were seen when comparing the osteo-mESCs and the calvarial cells. This study demonstrates that while osteo-mESCs share phenotypic characteristics with primary osteoblasts, there are some distinct differences in their biochemistry and response to cytokines. This is relevant to understanding differentiation of stem cells, developing in vitro models of disease, testing new drugs and developing cell therapies.An additional objective in this investigation was to look at tissue engineering strategies as a means of controlling inflammation in bone disease. The primary calvarial osteoblasts were utilised as an in vitro inflammation model, and used to study the effects of anti-inflammatory mediators. Anti-inflammatory-releasing porous scaffolds were manufactured from poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG). The calvarial osteoblast inflammation model was used successfully to show successful release of diclofenac sodium from the PLGA/PEG scaffolds. This study demonstrates that there is much to consider in the development of regenerative strategies for bone disease, particularly the role that the effect and control of inflammation will play in bone healing." @default.
• W1561261068 created "2016-06-24" @default.
• W1561261068 creator A5089113504 @default.
• W1561261068 date "2013-07-10" @default.
• W1561261068 modified "2023-09-27" @default.
• W1561261068 title "Tissue engineering in hostile environments : the effects and control of inflammation in bone tissue engineering" @default.
• W1561261068 cites W123538789 @default.
• W1561261068 cites W1475713056 @default.
• W1561261068 cites W1522174768 @default.
• W1561261068 cites W1534731743 @default.
• W1561261068 cites W1552674655 @default.
• W1561261068 cites W1578480468 @default.
• W1561261068 cites W158364933 @default.
• W1561261068 cites W1587777046 @default.
• W1561261068 cites W1588586232 @default.
• W1561261068 cites W1591990543 @default.
• W1561261068 cites W1595494026 @default.
• W1561261068 cites W1599568627 @default.
• W1561261068 cites W1686409300 @default.
• W1561261068 cites W1832914678 @default.
• W1561261068 cites W1898807996 @default.
• W1561261068 cites W1905412213 @default.
• W1561261068 cites W1908739351 @default.
• W1561261068 cites W1925667723 @default.
• W1561261068 cites W1935912749 @default.
• W1561261068 cites W1964001112 @default.
• W1561261068 cites W1964474592 @default.
• W1561261068 cites W1964912726 @default.
• W1561261068 cites W1965425745 @default.
• W1561261068 cites W1966198985 @default.
• W1561261068 cites W1967218254 @default.
• W1561261068 cites W1968351912 @default.
• W1561261068 cites W1971925267 @default.
• W1561261068 cites W1972098727 @default.
• W1561261068 cites W1972455777 @default.
• W1561261068 cites W1973898642 @default.
• W1561261068 cites W1975165380 @default.
• W1561261068 cites W1976841774 @default.
• W1561261068 cites W1977175847 @default.
• W1561261068 cites W1977280345 @default.
• W1561261068 cites W1977289618 @default.
• W1561261068 cites W1977950302 @default.
• W1561261068 cites W1978852166 @default.
• W1561261068 cites W1979013819 @default.
• W1561261068 cites W1980051005 @default.
• W1561261068 cites W1980183393 @default.
• W1561261068 cites W1982141317 @default.
• W1561261068 cites W1982512584 @default.
• W1561261068 cites W1985235031 @default.
• W1561261068 cites W1985310530 @default.
• W1561261068 cites W1985525204 @default.
• W1561261068 cites W1985557214 @default.
• W1561261068 cites W1986917247 @default.
• W1561261068 cites W1988212181 @default.
• W1561261068 cites W1989106205 @default.
• W1561261068 cites W1989194294 @default.
• W1561261068 cites W1989479258 @default.
• W1561261068 cites W1989634201 @default.
• W1561261068 cites W1990227092 @default.
• W1561261068 cites W1990463170 @default.
• W1561261068 cites W1990681926 @default.
• W1561261068 cites W1991354830 @default.
• W1561261068 cites W1992273666 @default.
• W1561261068 cites W1992324495 @default.
• W1561261068 cites W1992371993 @default.
• W1561261068 cites W1993399752 @default.
• W1561261068 cites W1994529997 @default.
• W1561261068 cites W1994596418 @default.
• W1561261068 cites W1994812709 @default.
• W1561261068 cites W1995063875 @default.
• W1561261068 cites W1995618846 @default.
• W1561261068 cites W1996752928 @default.
• W1561261068 cites W1997103341 @default.
• W1561261068 cites W1997812904 @default.
• W1561261068 cites W1997956124 @default.
• W1561261068 cites W1998086294 @default.
• W1561261068 cites W1999242737 @default.
• W1561261068 cites W1999422894 @default.
• W1561261068 cites W2000751851 @default.
• W1561261068 cites W2001294602 @default.
• W1561261068 cites W2002190966 @default.
• W1561261068 cites W2002315967 @default.
• W1561261068 cites W2003986577 @default.
• W1561261068 cites W2004337957 @default.
• W1561261068 cites W2004554331 @default.
• W1561261068 cites W2004719531 @default.
• W1561261068 cites W2005231713 @default.
• W1561261068 cites W2005528887 @default.
• W1561261068 cites W2005972147 @default.
• W1561261068 cites W2006712783 @default.
• W1561261068 cites W2006955799 @default.
• W1561261068 cites W2007452107 @default.
• W1561261068 cites W2010067507 @default.
• W1561261068 cites W2010226911 @default.
• W1561261068 cites W2010824264 @default.
• W1561261068 cites W2010893406 @default.
• W1561261068 cites W2011553184 @default.
• W1561261068 cites W2011653132 @default.
• W1561261068 cites W2012275514 @default.
• W1561261068 cites W2012306000 @default.

• next