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- W1561738596 abstract "The serine-specific amber suppressor tRNA of Escherichia coli cells carrying the suI+ gene—a minor serine-tRNA species—has been partially purified. In vitro studies with the tRNA obtained show binding of seryl-tRNA to ribosomes in the presence of UAG and also poly rUAG-dependent polyserine formation. In in vitro protein synthesis experiments with RNA of an amber mutant of bacteriophage f2 as messenger, 100% suppression could be achieved by increasing the concentration of the purified suppressor tRNA. Taking a decrease in suppression in this system as an assay for chain termination, no evidence for a chain-terminator tRNA was obtained. Crude tRNA of E. coli cells carrying the amber suppressor gene suIII+ showed in vitro suppression in f2am RNA-directed protein synthesis." @default.
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- W1561738596 date "1968-05-01" @default.
- W1561738596 modified "2023-10-16" @default.
- W1561738596 title "Studies on polynucleotides" @default.
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- W1561738596 doi "https://doi.org/10.1016/0022-2836(68)90243-x" @default.
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