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- W1562055868 abstract "A chromosomal fragment of 6.7 megadaltons (MDa), apparently containing the genes for milk protein utilization by Streptococcus lactis subsp. lactis SSL135, was cloned in S. lactis subsp. lactis MG1614, a proteinase-negative strain. For the cloning, the chromosomal DNA of SSL135 was cleaved with restriction enzyme Bam HI and the resulting fragments were ligated to the single Bcl I site of pVS2, a 3.3-MDa chloramphenicol-erythromycin double-resistance plasmid constructed in this laboratory. S. lactis subsp. lactis MG1614 was transformed by using this ligation mixture and selecting for chloramphenicol resistance and growth in citrated milk medium. One clone containing a 10.0-MDa plasmid, subsequently designated as pVS6, was chosen for further studies. Despite the lack of homology with previously characterized proteinase genes of lactic streptococci, the cloned insert consistently conveyed the ability to grow in milk to proteinase-negative recipients in repeated transformation experiments. The genetic evidence suggests that the main part of the gene(s) for the proposed proteinase activity is located within a 3.8-MDa Bgl II fragment of the clone." @default.
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- W1562055868 date "1987-07-01" @default.
- W1562055868 modified "2023-10-15" @default.
- W1562055868 title "Cloning of a <i>Streptococcus lactis</i> subsp. <i>lactis</i> Chromosomal Fragment Associated with the Ability To Grow in Milk" @default.
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- W1562055868 doi "https://doi.org/10.1128/aem.53.7.1584-1588.1987" @default.
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