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• W1562513141 abstract "The effects of Cu amendment on indigenous soil microorganisms were investigated in two soils, a calcareous silty clay (Ep) and a sandy soil (Au), by means of a 1-year field experiment and a two-month microcosm incubation. Cu was added as ‘Bordeaux mixture’ [CuSO4, Ca(OH)2] at the standard rate used in viticulture (B1 = 16 kg Cu kg−1 soil) and at a higher level of contamination (B3=48 kg Cu ha−1 soil). More extractable Cu was observed in sandy soil (Au) than in silty soil (Ep). Furthermore, total Cu and Cu-EDTA declined with time in Au soil, whereas they remained stable in Ep soil. Quantitative modifications of the microflora were assessed by C-biomass measurements and qualitative modifications were assessed by the characterization of the genetic structure of bacterial and fungal communities from DNA directly extracted from the soil, using B- and F-ARISA (bacterial and fungal automated ribosomal intergenic spacer analysis). In the field study, no significant modifications were observed in C-biomass whereas microcosm incubation showed a decrease in B3 contamination only. ARISA fingerprinting showed slight but significant modifications of bacterial and fungal communities in field and microcosm incubation. These modifications were transient in all cases, suggesting a short-term effect of Cu stress. Microcosm experiments detected the microbial community modifications with greater precision in the short-term, while field experiments showed that the biological effects of Cu contamination may be overcome or hidden by pedo-climatic variations." @default.
• W1562513141 created "2016-06-24" @default.
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• W1562513141 date "2006-11-01" @default.
• W1562513141 modified "2023-09-23" @default.
• W1562513141 title "Field and microcosm experiments to evaluate the effects of agricultural Cu treatment on the density and genetic structure of microbial communities in two different soils" @default.
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• W1562513141 doi "https://doi.org/10.1111/j.1574-6941.2006.00157.x" @default.
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